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Gene Expression Data
Assay Details
Assay
Reference: J:229480 Baker JL, et al., Testicular receptor 2, Nr2c1, is associated with stem cells in the developing olfactory epithelium and other cranial sensory and skeletal structures. Gene Expr Patterns. 2016 Jan;20(1):71-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5795684
Gene symbol: Nr2c1
Gene name: nuclear receptor subfamily 2, group C, member 1
Antibody: Anti-NR2C1 Antibody
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 2A/C/D/E: embryonic day 10.5 (more )
Note: Double labeled: red - Nr2c1; green - Pax7 (assay MGI:5795696).
Structure Level Pattern Image Note
TS17: olfactory epithelium Present Punctate 2E , 2D , 2C , 2A Concentrated in puncta within the nucleus. A subset of Nr2c1-expressing cells coexpress Pax7.

Specimen 2F/H/I/J: embryonic day 10.5 (more )
Note: Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
Structure Level Pattern Image Note
TS17: olfactory epithelium Present 2J , 2H , 2I , 2F Numerous Nr2c1-expressing cells co-express Ascl1.

Specimen 2K/L/O: embryonic day 10.5 (more )
Note: Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
Structure Level Pattern Image Note
TS17: olfactory migratory mass Present 2M , 2L , 2N , 2K Some Ncam1-positive neurons in the migratory mass show Nr2c1 expression.
TS17: olfactory epithelium Strong 2P , 2O , 2K , 2Q Cells that robustly express Nr2c1 are not co-labeled with Ncam1, and Ncam1-labeled cells express Nr2c1 at low levels.

Specimen 3A/C/D: embryonic day 11.5 (more )
Note: Double labeled: red - Nr2c1; green - Pax7 (assay MGI:5795696).
Structure Level Pattern Image Note
TS19: olfactory epithelium Present Widespread 3C , 3A , 3D Expressed throughout, but more robust in the medial versus lateral region. No longer robustly associated with Pax7 labeled olfactory stem cells.

Specimen 3F/H/I/J: embryonic day 11.5 (more )
Note: Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
Structure Level Pattern Image Note
TS19: olfactory epithelium Present Widespread 3I , 3J , 3F , 3H Expressed throughout, but more robust in the medial versus lateral region. In the medial olfactory epithelium frequently coexpressed with Ascl1. Expression is primarily cytoplasmic.

Specimen 3K/M/N/P: embryonic day 11.5 (more )
Note: Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
Structure Level Pattern Image Note
TS19: olfactory epithelium Present Widespread 3N , 3P , 3M , 3K Expressed throughout, but more robust in the medial versus lateral region. Most Ncam1 positive cells do not express Nr2c1.

Specimen 4A/B: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: olfactory epithelium Strong Widespread 4A , 4B Expressed in both the thicker, presumptive neural epithelium, and in the thinner, presumptive respiratory epithelium. Primarily cytoplasmic.

Specimen 4C: embryonic day 16.5 (more )
Note: Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
Structure Level Pattern Image Note
TS24: olfactory epithelium Strong 4C Expressed in small subsets of Ascl1-positive cells.

Specimen 4D: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: vomeronasal organ Present 4D Expression is less robust than it is in the adjacent olfactory epithelium.
TS22: olfactory epithelium Present 4D

Specimen 4E: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: vomeronasal organ Present 4E Expression is less robust than it is in the adjacent olfactory epithelium.
TS25: olfactory epithelium Present 4E

Specimen 4F: embryonic day 11.5 (more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Structure Level Pattern Image Note
TS19: forebrain ventricular layer Present Regionally restricted 4F inset , 4F Expressed in the anterior forebrain, the presumed olfactory bulb rudiment.
TS19: telencephalon Weak Regionally restricted 4F inset , 4F Expressed in the anterior dorsal region.

Specimen 4G: embryonic day 14.5 (more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Structure Level Pattern Image Note
TS22: main olfactory bulb Absent 4G inset , 4G

Specimen 5A/B: embryonic day 10.5 (more )
Note: Double labeled: red - Nr2c1; green - Tubb3 (assay MGI:5795701).
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 5A , 5B
TS17: otocyst Present 5A , 5B

Specimen 5C: embryonic day 16.5 (more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Structure Level Pattern Image Note
TS24: vibrissa Strong Regionally restricted 5C Heavily expressed in the supporting cells of the mystacial vibrissae.

Specimen 5D: embryonic day 16.5 (more )
Note: Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
Structure Level Pattern Image Note
TS24: vibrissa Strong Regionally restricted 5D Heavily expressed in the supporting cells of the mystacial vibrissae. But not coincided with the NCAM1-positive neural processes that innervate the vibrissae.

Specimen 5E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: pigmented retinal epithelium Present 5E inset , 5E
TS22: neural retina Weak Regionally restricted 5E Largely absent. Low-level diffuse signal in the ciliary marginal zone.

Specimen 6A/B/C: embryonic day 17.5 (more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Structure Level Pattern Image Note
TS25: tooth mesenchyme Strong Regionally restricted 6A , 6B Expressed in the dental pulp. Expressed in the vicinity of, and co-localized with a subset of Nestin-positive presumptive dental mesenchymal stem cells.
TS25: tooth Strong Regionally restricted 6A , 6C Expressed in a subset of presumptive odontoblasts at the margin of the dental pulp.

Specimen 6D: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: jaw Present Regionally restricted 6D Expressed in the mesenchymal stem cells and presumptive osteoblasts in the developing alveolar bone.

Specimen 6E/F/G: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: nasal septum cartilage Present 6E , 6F Expressed in the presumptive septal bone.
TS25: viscerocranium bone Present Regionally restricted 6G , 6E Expressed in the presumptive bone of the palatine process.

Specimen S1C: embryonic day 17.5 (more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Structure Level Pattern Image Note
TS25: head Strong S1C Expressed in multiple craniofacial structures.

Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forebrain Trace Diffuse Limited hardly above background expression in the cortex, ventral forebrain structures, and thalamus.

Specimen not shown: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: forebrain Trace Diffuse Limited hardly above background expression in the cortex, ventral forebrain structures, and thalamus.
TS22: cerebral cortex Trace Diffuse Limited hardly above background expression.
TS22: thalamus Trace Diffuse Limited hardly above background expression.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
09/17/2024
MGI 6.24
The Jackson Laboratory