Col6a3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:208903 Pan TC, et al., A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16. J Biol Chem. 2014 Apr 11;289(15):10293-307
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5804160
Gene symbol:	Col6a3
Gene name:	collagen, type VI, alpha 3

Antibody:	collagen VI alpha3 antibody
Detection system:	Cy3

Results

5Aa ©

Specimen 5Aa: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: limb	Present		5Aa	Fibrous expression pattern in dermis, skeletal muscle and around the ribs.

5Ab ©

Specimen 5Ab: embryonic day 17.0; Col6a3^tm2.1Chu/Col6a3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS25: limb	Strong		5Ab	Increased levels of expression in mutant limbs compared with wild-type.

5Ac ©

Specimen 5Ac: embryonic day 17.0; Col6a3^tm2.1Chu/Col6a3^tm2.1Chu (more

)

Structure	Level	Pattern	Image	Note
TS25: limb	Strong		5Ac	Increased levels of expression in mutant limbs compared with wild-type.

6A a3(VI) +/+ ©

Specimen 6A alpha3(VI) +/+: embryonic day 17.0 (more

)

Structure	Level	Image
TS25: dermis	Strong	6A a3(VI) +/+
TS25: rib	Strong	6A a3(VI) +/+
TS25: skeletal muscle	Strong	6A a3(VI) +/+

6A a3(VI) +/d16 ©

Specimen 6A alpha3(VI) +/d16: embryonic day 17.0; Col6a3^tm2.1Chu/Col6a3⁺ (more

)

Structure	Level	Image
TS25: dermis	Strong	6A a3(VI) +/d16
TS25: rib	Strong	6A a3(VI) +/d16
TS25: skeletal muscle	Strong	6A a3(VI) +/d16

6A a3(VI) d16/d16 ©

Specimen 6A alpha3(VI) d16/d16: embryonic day 17.0; Col6a3^tm2.1Chu/Col6a3^tm2.1Chu (more

)

Structure	Level	Image
TS25: dermis	Strong	6A a3(VI) d16/d16
TS25: rib	Strong	6A a3(VI) d16/d16
TS25: skeletal muscle	Strong	6A a3(VI) d16/d16

Specimen day 17 not shown +/+: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: lung	Strong

Specimen day 17 not shown +/d16: embryonic day 17.0; Col6a3^tm2.1Chu/Col6a3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS25: lung	Strong

Specimen day 17 not shown d16/d16: embryonic day 17.0; Col6a3^tm2.1Chu/Col6a3^tm2.1Chu (more

)

Structure	Level	Pattern	Image	Note
TS25: dermis	Strong
TS25: rib	Strong
TS25: skeletal muscle	Strong

6C a3(VI) +/+ ©

Specimen 6C alpha3(VI) +/+: postnatal week 2 (more

)

Structure	Level	Pattern	Image	Note
TS28: tail	Present	Regionally restricted	6C a3(VI) +/+	Expression in tendon fascicles, muscle, nerve and vertebrae. Less prominent expression in tendon sheaths.
TS28: tail vertebra	Present		6C a3(VI) +/+

6C a3(VI) +/d16 ©

Specimen 6C alpha3(VI) +/d16: postnatal week 2; Col6a3^tm2.1Chu/Col6a3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: tail	Present	Regionally restricted	6C a3(VI) +/d16	Expression in tendon fascicles, muscle, nerve and vertebrae. Less prominent expression in tendon sheaths.
TS28: tail vertebra	Present		6C a3(VI) +/d16

6C a3(VI) d16/d16 ©

Specimen 6C alpha3(VI) d16/d16: postnatal week 2; Col6a3^tm2.1Chu/Col6a3^tm2.1Chu (more

)

Structure	Level	Pattern	Image	Note
TS28: tail	Present	Regionally restricted	6C a3(VI) d16/d16	Expression in tendon fascicles, muscle, nerve and vertebrae. Less prominent expression in tendon sheaths.
TS28: tail vertebra	Present		6C a3(VI) d16/d16

7A a3(VI) +/+ ©

Specimen 7A alpha3(VI) +/+: postnatal month 1 (more

)

Structure	Level	Pattern	Image	Note
TS28: quadriceps femoris muscle	Present		7A a3(VI) +/+

Specimen 7A alpha3(VI) +/d16: postnatal month 1; Col6a3^tm2.1Chu/Col6a3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: quadriceps femoris muscle	Weak		7A a3(VI) +/d16	Expression somewhat decreased compared with wild-type muscle.

Specimen 7A alpha3(VI) d16/d16: postnatal month 1; Col6a3^tm2.1Chu/Col6a3^tm2.1Chu (more

)

Structure	Level	Pattern	Image	Note
TS28: quadriceps femoris muscle	Trace		7A a3(VI) d16/d16	Expression barely detectable.

9_a3(VI) +/+ ©

Specimen 9 alpha3(VI) +/+: postnatal month 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: quadriceps femoris muscle	Present		9_a3(VI) +/+

Specimen 9 alpha3(VI) +/d16: postnatal month 6; Col6a3^tm2.1Chu/Col6a3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: quadriceps femoris muscle	Weak		9_a3(VI) +/d16	Expression decreased compared with wild-type muscle.

Specimen 9 alpha3(VI) d16/d16: postnatal month 6; Col6a3^tm2.1Chu/Col6a3^tm2.1Chu (more

)

Structure	Level	Pattern	Image	Note
TS28: quadriceps femoris muscle	Weak		9_a3(VI) d16/d16	Expression decreased compared with wild-type muscle.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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