Gnb4tm1.1(cre)Hze
Targeted Allele Detail
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| Symbol: |
Gnb4tm1.1(cre)Hze |
| Name: |
guanine nucleotide binding protein (G protein), beta 4; targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:5806633 |
| Synonyms: |
Gnb4-IRES2-Cre-D |
| Gene: |
Gnb4 Location: Chr3:32634481-32670734 bp, - strand Genetic Position: Chr3, 15.78 cM, cytoband B
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| Alliance: |
Gnb4tm1.1(cre)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Gnb4tm1.1(cre)Hze expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a partial Gnb4 sequence spanning intron 6 through intron 9, an frt3 site within Gnb4 intron 9, a partial Gnb4 exon 10 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the the AttB/AttP-flanked PGK-neo sequence and replace it with the recombined AttB/AttP site (AttL).
(J:236597)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Gnb4
(mouse)
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| Original: |
J:236597 Wang Q, et al., Organization of the connections between claustrum and cortex in the mouse. J Comp Neurol. 2017 Apr 15;525(6):1317-1346 |
| All: |
3 reference(s) |
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Analysis Tools
