Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119664
Houde C, et al., Hippi is essential for node cilia assembly and Sonic hedgehog signaling. Dev Biol. 2006 Dec 15;300(2):523-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5807056
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal probe21
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4A:
embryonic day 8.0
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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involves: 129S1/SvImJ * C57BL/6J
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/SvImJ * C57BL/6J
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Mutant Allele(s):
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Ift57tm1Dwni/Ift57+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Strong |
Regionally restricted |
4B
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Expression is asymmetrical. Strong expression in the left lateral plate mesoderm. Authors state that heterozygotes always stain as the wildtype embryos.
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| TS12: node |
Present |
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4B
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Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/SvImJ * C57BL/6J
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Mutant Allele(s):
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Ift57tm1Dwni/Ift57tm1Dwni
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Strong |
Regionally restricted |
4C
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Expression is detected bilaterally in both the left and right lateral plate mesoderm.
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| TS12: node |
Weak |
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4C
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Downregulation of expression around the node region compared to wildtype and heterozygous embryos.
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Analysis Tools