Tg(CYP19A1-icre)3Fcrt
Transgene Detail
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| Symbol: |
Tg(CYP19A1-icre)3Fcrt |
| Name: |
transgene insertion 3, Sophie Fouchecourt |
| MGI ID: |
MGI:5807485 |
| Synonyms: |
ARO-iCre, aromatase-iCre |
| Transgene: |
Tg(CYP19A1-icre)3Fcrt Location: unknown
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| Alliance: |
Tg(CYP19A1-icre)3Fcrt page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Humanized sequence, Recombinase) |
| Mutation: |
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Insertion
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Tg(CYP19A1-icre)3Fcrt expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
CYP19A1 (1588) |
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Mutation details: The transgene contains the ovary- and testis-specific promoter of the human cytochrome P450, family 19, subfamily a, polypeptide 1 (CYP19A1) gene, comprising 278 base pairs (bp) of 5' flanking DNA and 26 bp of the alternatively spliced exon IIa, driving expression of mammalian codon-improved Cre recombinase (icre); the coding sequence is followed by the SV40 polyadenylation signal. RT-PCR analysis of various tissues (heart, muscle, lung, spleen, brain, liver, pancreas, ovary, testis) demonstrates Cre recombinase-mediated excision of a loxP-flanked sequence only in the ovaries and testes of transgenic mice. Histologic analysis of X-gal-stained testes of mice carrying this transgene and a lacZ Cre reporter allele (Gt(ROSA)26Sortm1Sor) reveal Cre recombinase activity from postpartum day 3 (P3) in Sertoli cells at the periphery of the seminiferous tubules and in the Leydig cells of the interstitium, but not in germ cells.
(J:229626)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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CYP19A1
(human)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:229626 Fumel B, et al., Expression of dominant-negative thyroid hormone receptor alpha1 in Leydig and Sertoli cells demonstrates no additional defect compared with expression in Sertoli cells only. PLoS One. 2015;10(3):e0119392 |
| All: |
1 reference(s) |
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Analysis Tools
