Probe:	Wnk1/Hsn2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The probe detects sensory associated splice isoform, Wnk1/Hsn2. Semi-quantitative evaluation was summarized in Table 1, 2, 3 and 4.

Structure	Level	Pattern	Image	Note
TS17: brain	Weak	Ubiquitous	1C upper
TS17: future spinal cord	Weak	Ubiquitous	1C upper
TS17: heart	Trace	Ubiquitous	1C upper	Very weak.
TS17: liver	Trace	Ubiquitous	1C upper	Very weak.
TS17: spinal ganglion	Trace	Ubiquitous	1C upper	Very weak.
TS17: brain ventricular layer	Present	Ubiquitous	1C upper

Structure	Level	Pattern	Image	Note
TS20: brain	Weak	Ubiquitous	1C lower
TS20: liver	Weak	Ubiquitous	1C lower
TS20: spinal ganglion	Weak	Ubiquitous	1C lower
TS20: spinal cord	Strong	Ubiquitous	1C lower	Stronger expression than Wnk1/Hsn2. Greater expression than the rest of the developing embryo.
TS20: heart	Trace	Ubiquitous	1C lower	Very weak.
TS20: brain ventricular layer	Present	Ubiquitous	1C lower

Structure	Level	Pattern	Image	Note
TS23: brain	Weak	Uniform	2C	Stronger expression than Wnk1.
TS23: spinal cord	Strong	Uniform	2C	Stronger expression than Wnk1.
TS23: spinal ganglion	Moderate	Uniform	2C	Stronger expression than Wnk1.
TS23: heart	Trace	Uniform	2C	Very weak.
TS23: liver	Moderate	Uniform	2C
TS23: dorsal root ganglion	Strong	Uniform	2C	Stronger expression than Wnk1.
TS23: olfactory epithelium	Strong	Uniform	2C	Stronger expression than Wnk1.
TS23: trigeminal ganglion	Strong	Uniform	2C	Stronger expression than Wnk1.

Structure	Level	Pattern	Image	Note
TS27: olfactory epithelium	Strong	Non-Uniform	2G	Heterogeneousm distribution.
TS27: trigeminal ganglion	Weak	Non-Uniform	2G
TS27: heart	Trace	Non-Uniform	2G	Very weak.
TS27: liver	Trace	Non-Uniform	2G	Very weak.
TS27: lung	Trace	Non-Uniform	2G	Very weak.
TS27: metanephros	Moderate	Non-Uniform	2G
TS27: spinal ganglion	Moderate	Non-Uniform	2G
TS27: thymus	Moderate	Non-Uniform	2G
TS27: brain	Moderate	Non-Uniform	2G	Stronger expression than Wnk1.
TS27: spinal cord	Moderate	Non-Uniform	2G	Stronger expression than Wnk1.
TS27: intervertebral disc	Strong		2G	Stronger expression than Wnk1.

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		3C	Stronger expression than Wnk1.
TS27: spinal cord	Present	Regionally restricted	3C	Expressed in the ventral, ventromedial and medial nuclei. Stronger expression than Wnk1.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Strong		4C

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Strong	Regionally restricted	4D	Expressed in the dorsal root.

Structure	Level	Pattern	Image	Note
TS27: myenteric nerve plexus	Present		5B	Stronger expression than Wnk1.

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Present		5E
TS28: habenula	Present		5E

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Moderate	Non-Uniform	6C	Heterogeneousm distribution.
TS28: brain	Strong	Non-Uniform	6C
TS28: spinal cord	Strong	Non-Uniform	6C
TS28: thymus	Strong	Non-Uniform	6C
TS28: metanephros	Moderate	Non-Uniform	6C
TS28: spinal ganglion	Moderate	Non-Uniform	6C
TS28: spleen	Moderate	Non-Uniform	6C
TS28: heart	Trace	Non-Uniform	6C	Very weak.
TS28: liver	Trace	Non-Uniform	6C	Very weak.
TS28: lung	Absent		6C
TS28: trigeminal ganglion	Absent		6C

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Strong	Non-Uniform	6G	Heterogeneousm distribution.
TS28: brain	Strong	Non-Uniform	6G
TS28: metanephros	Weak	Non-Uniform	6G
TS28: spleen	Weak	Non-Uniform	6G
TS28: testis	Weak	Non-Uniform	6G
TS28: spinal cord	Moderate	Non-Uniform	6G
TS28: spinal ganglion	Moderate	Non-Uniform	6G
TS28: thymus	Moderate	Non-Uniform	6G
TS28: trigeminal ganglion	Moderate	Non-Uniform	6G
TS28: heart	Absent		6G
TS28: liver	Absent		6G
TS28: lung	Absent		6G

Structure	Level	Pattern	Image	Note
TS28: hair root sheath matrix	Weak		7B
TS28: hair inner root sheath	Absent		7B
TS28: hair outer root sheath	Absent		7B
TS28: hair medulla	Moderate		7B	Stronger expression than Wnk1.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		8C
TS28: spinal nerve	Present	Regionally restricted	8C	Much less expression in the ventral roots, predominated in spinal ganglia neuronal cells.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present	Regionally restricted	9E	Detected in the somata of the neurons and the supporting satellite cells.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present		9H

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		10C	Increased expression.
TS28: hippocampus CA3	Present		10C	Increased expression.
TS28: hippocampus CA1	Present		10C

Structure	Level	Pattern	Image	Note
TS28: distal convoluted tubule	Strong		11B
TS28: glomerular tuft	Ambiguous		11B	Little to no labeling detected.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Ambiguous		11E	Little to no labeling detected.
TS28: distal convoluted tubule	Strong		11E

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Weak		12C	Very low expression in the wall.
TS28: spermatogonium	Weak		12C	Very low.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	S2E	Detected in the somata of the neurons and the supporting satellite cells.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Strong		S2H	High expression in the supporting satellite cells. Also expressed in the neuronal somata.