Gene Expression Data
Assay Details
Assay
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Reference:
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J:80863
Crawley JT, et al., Distribution of endothelial cell protein C/activated protein C receptor (EPCR) during mouse embryo development. Thromb Haemost. 2002 Aug;88(2):259-66
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5811401
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Gene symbol:
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Procr
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Gene name:
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protein C receptor, endothelial
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Antibody:
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Anti-EPCR 1560
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Detection system:
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Secondary antibody coupled to Texas Red
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Assay notes:
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Three antibodies were described; the one used in this assay was not specified. MGI:5811364, MGI:5811365, or MGI:581363 could have been used.
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Results
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Specimen
1A embryonic:
embryonic day 7.5
(more )
Specimen
1A embryonic:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1A maternal:
postnatal adult
(more )
Specimen
1A maternal:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: vasculature of uterus |
Present |
Regionally restricted |
1A
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Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
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TS28: venous system endothelium |
Present |
Regionally restricted |
1A
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Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
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TS28: decidua |
Present |
Non-Uniform |
1A
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Decidual cells in the compact layer stained variably, whereas those in the spongy layer exhibited little or no staining.
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Specimen
1C embryonic:
(close )
Genetic Background:
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either: 129S/SvEv-Procrtm1Cte or (involves: 129S7/SvEvBrd * Black Swiss)
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Mutant Allele(s):
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Procrtm1Cte/Procrtm1Cte
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Age:
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embryonic day 7.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1C maternal:
postnatal adult; Procrtm1Cte/Procr +
(more )
Specimen
1C maternal:
(close )
Genetic Background:
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either: 129S/SvEv-Procrtm1Cte or (involves: 129S7/SvEvBrd * Black Swiss)
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Mutant Allele(s):
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Procrtm1Cte/Procr+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: vasculature of uterus |
Present |
Regionally restricted |
1C
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Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
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TS28: venous system endothelium |
Present |
Regionally restricted |
1C
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Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
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Specimen
1F embryonic:
embryonic day 9.5
(more )
Specimen
1F embryonic:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1F maternal:
postnatal adult
(more )
Specimen
1F maternal:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: vasculature of uterus |
Strong |
Regionally restricted |
1F
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Expression was strong in the endothelial cells lining the venous sinusoids.
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TS28: venous system endothelium |
Strong |
Regionally restricted |
1F
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Expression was strong in the endothelial cells lining the venous sinusoids.
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TS28: decidua |
Strong |
Regionally restricted |
1F
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Expression was detected in the decidual cells of the compact layer, but the cells in the spongy layer were negative.
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Specimen
2F:
embryonic day 13.5
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
2F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
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Specimen
2G:
embryonic day 14.5
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
2G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
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Specimen
2H:
embryonic day 15.5
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
2H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
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Specimen
3C:
embryonic day 16.5
(more )
Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3D:
embryonic day 16.5
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E:
embryonic day 20.5
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
3E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 20.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: blood vessel endothelium |
Present |
Regionally restricted |
3E
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Expression was detected in the endothelium of some vessels in close proximity to the ribs.
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TS26: aorta |
Present |
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3E
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Expression was confined to the endothelial cells.
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TS26: vena cava |
Present |
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3E
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Expression was confined to the endothelial cells.
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Specimen
3F:
postnatal day 1
(more )
Specimen
3F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3G:
postnatal adult
(more )
Specimen
3G:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3H:
postnatal adult
(more )
Specimen
3H:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A:
postnatal day 1
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4A:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Specimen
4B:
postnatal adult
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lung vascular element |
Strong |
Regionally restricted |
4B
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The larger vessels of the lung, but not the alveolar capillaries, stained strongly.
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Specimen
4C:
postnatal day 1
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 1
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Specimen
4D:
postnatal adult
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: liver vasculature |
Present |
Regionally restricted |
4D
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Expression was detected in the large vessels and sinusoidal capillaries.
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TS28: hepatic artery |
Present |
|
4D
|
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TS28: hepatic vein |
Present |
Regionally restricted |
4D
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Staining was detected in the enothelium of central veins.
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Specimen
4E:
postnatal day 1
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: renal artery |
Moderate |
Regionally restricted |
4E
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Staining was observed in the largest arteries and veins, but not the smaller vessels.
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TS27: renal vein |
Moderate |
Regionally restricted |
4E
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Staining was observed in the largest arteries and veins, but not the smaller vessels.
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TS27: glomerular capillary system |
Absent |
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4E
|
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Specimen
4F:
postnatal adult
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: renal artery |
Moderate |
Regionally restricted |
4F
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Staining was observed in the largest arteries and veins, but not the smaller vessels.
|
TS28: renal vein |
Moderate |
Regionally restricted |
4F
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Staining was observed in the largest arteries and veins, but not the smaller vessels.
|
TS28: glomerular capillary system |
Absent |
|
4F
|
|
Specimen
4G:
postnatal day 1
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: brain blood vessel |
Present |
Regionally restricted |
4G
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Expression was detected in the larger vessels, but not capillaries.
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Specimen
4H:
postnatal adult
(more )
Note: Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
Specimen
4H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Procr; green - Pecam1 (assay MGI:5811556).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain blood vessel |
Present |
Regionally restricted |
4H
|
Expression was detected in the larger vessels, but not capillaries.
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