Mki67<tm2.1(cre/ERT2)Cle> Targeted Allele Detail MGI Mouse (MGI:5816737)

Mki67^{tm2.1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

Symbol:	Mki67^{tm2.1(cre/ERT2)Cle}
Name:	antigen identified by monoclonal antibody Ki 67; targeted mutation 2.1, Hans Clevers
MGI ID:	MGI:5816737
Synonyms:	Ki67^iresCreEr
Gene:	Mki67 Location: Chr7:135291513-135318090 bp, - strand Genetic Position: Chr7, 81.32 cM, cytoband F3-F5
Alliance:	Mki67^{tm2.1(cre/ERT2)Cle} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:254891
Parent Cell Line:	IB10/E14IB10 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Mki67^{tm2.1(cre/ERT2)Cle} expression driven by 1 gene

Mutation details: A targeting vector containing ires2-creERT2 and SV40 polyadenylation site fllowed by a FRT site flanked PGKneo cassette was used to introduce cre/ERT2 at the C-terminus of the Mki67 gene. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:254891)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mki67 (mouse) Summary of all recombinase alleles driven by Mki67. Alliance mouse Mki67 gene page

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Mki67 Mutation:	146 strains or lines available

References

Original:	J:254891 Basak O, et al., Troy+ brain stem cells cycle through quiescence and regulate their number by sensing niche occupancy. Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):E610-E619
All:	10 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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