Gene Expression Data
Assay Details
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Assay
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Reference:
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J:199559
Manning DK, et al., Loss of the ciliary kinase Nek8 causes left-right asymmetry defects. J Am Soc Nephrol. 2013 Jan;24(1):100-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5817984
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal probe24
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 8.0-8.5
(more  )
Note: 4-5 somites
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Non-Uniform |
3A
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Expression is stronger on the left side.
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3A
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Expressed in the left lateral plate mesoderm.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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Specimen
S1A:
embryonic day 8.0-8.5
(more )
Note: 4-5 somites
Specimen
S1A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Non-Uniform |
S1A
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Expression is stronger on the left side.
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Specimen
S1B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Uniform |
S1B
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Symmetrical nodal expression.
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Specimen
S1C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Uniform |
S1C
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Symmetrical nodal expression.
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Specimen
S1D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Uniform |
S1D
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Symmetrical nodal expression.
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Analysis Tools
