Gene Expression Data
Assay Details
Assay
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Reference:
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J:15430
Wada J, et al., Cloning of cDNA for the alpha subunit of mouse insulin-like growth factor I receptor and the role of the receptor in metanephric development. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10360-4
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5818281
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Gene symbol:
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Igf1r
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Gene name:
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insulin-like growth factor I receptor
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Probe:
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Igf1r cDNA5
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Probe preparation:
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Antisense labelled with S35 DNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A:
embryonic day 13.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: metanephros |
Present |
Regionally restricted |
3A
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Expression was detected throughout the parenchyma of the kidney.
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Specimen
3B:
embryonic day 13.5
(more )
Specimen
3B:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: metanephros |
Present |
Regionally restricted |
3B
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Expression was heavily concentrated in the ureter bud, its branches, and the tips of the branches.
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Specimen
3C:
postnatal day 2
(more )
Specimen
3C:
(close )
Genetic Background:
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Swiss Webster
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS27: metanephros |
Present |
Regionally restricted |
3C
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Expression was reduced. Expression was sparsely distributed over the parenchyma of the kidney.
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TS27: renal tubule |
Present |
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3C
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Expression was relatively concentrated over the tubules in comparison to the glomeruli.
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TS27: glomerular tuft |
Ambiguous |
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3C
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