Probe:	pLhx3-delta
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Present		3A
TS15: neural tube	Present	Regionally restricted	3A	Expressed as two stripes in the ventral spinal cord extending bilaterally in a caudal direction posterior to the otic vesicles.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		3B
TS17: future spinal cord	Present		3B
TS17: embryo	Present	Regionally restricted	3B	Expressed in structures immediately anterior-dorsal of the otic vesicles.
TS17: hindbrain	Present	Regionally restricted	3B	Expression extended in an anterior direction to ventro-lateral fields.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		3C
TS17: embryo	Present	Regionally restricted	3C	Expressed in structures immediately anterior-dorsal of the otic vesicles.
TS17: hindbrain	Present	Regionally restricted	3C	Expression extended in an anterior direction to ventro-lateral fields.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		3D , 3E	Expressed in both the pars distalis and pars intermedia.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		3F	Expressed in both the pars distalis and pars intermedia.

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	3G	Expressed in the ventro-lateral regions. No expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	3H	Expressed in a more extensive array near the floor plate.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3I	Expressed as two symmetrical ventro-lateral columns.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent