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Gene Expression Data
Assay Details
Assay
Reference: J:85925 Moreau-Fauvarque C, et al., The transmembrane semaphorin Sema4D/CD100, an inhibitor of axonal growth, is expressed on oligodendrocytes and upregulated after CNS lesion. J Neurosci. 2003 Oct 8;23(27):9229-39
Assay type: RNA in situ
MGI Accession ID: MGI:5882750
Gene symbol: Sema4d
Gene name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Probe: Sema4d cDNA3
Probe preparation: Antisense labelled with fluorescein RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1D: postnatal day 10 (more )
Note: Double labeled: red - Sema4d; blue - Pdgfra (assay MGI:5882749).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 1D Expression was detected in oligodendrocytes in the cerebellum. No more than 5% of the labeled oligodendrocytes coexpressed Pdgfra, which is mainly expressed at early stages of oligodendrocyte development.
Specimen 1E: postnatal day 10 (more )
Note: Double labeled: red - Sema4d; blue - Olig1 (assay MGI:5882751).
Structure Level Pattern Image Note
TS28: corpus callosum Present Regionally restricted 1E A high level of expression was noted. 95% of the labeled cells also expressed Olig1.
Specimen 1F: postnatal day 10 (more )
Note: Double labeled: red - Sema4d; blue - Olig2 (assay MGI:5882752).
Structure Level Pattern Image Note
TS28: corpus callosum Present Regionally restricted 1F A high level of expression was noted. 95% of the labeled cells also expressed Olig2, which represented ~20% of the Olig2-positive population.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory