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Pdgfrbtm1.1(cre/ERT2)Csln
Targeted Allele Detail
Summary
Symbol: Pdgfrbtm1.1(cre/ERT2)Csln
Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 1.1, Chyuan-Sheng Lin
MGI ID: MGI:5901894
Synonyms: PDGFRbata-P2A-CreERT2
Gene: Pdgfrb  Location: Chr18:61178222-61218133 bp, + strand  Genetic Position: Chr18, 34.41 cM
Alliance: Pdgfrbtm1.1(cre/ERT2)Csln page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:263753
Parent Cell Line:  KV1 (ES Cell)
Strain of Origin:  (C57BL/6J x 129S6/SvEvTac)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Pdgfrbtm1.1(cre/ERT2)Csln expression driven by 1 gene
 
Mutation details: A targeting vector was designed to replace the translation stop codon of the platelet derived growth factor receptor, beta polypeptide (Pdgfrb) gene with a creER fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) followed by a frt-flanked neomycin resistance (neo) cassette. A viral P2A oligopeptide, from porcine teschovirus-1, was inserted upstream of the creER fusion gene to ribosomal skipping. The construct was electroporated into (C57BL/6J x 129S6/SvEvTac)F1-derived KV1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL6/N blastocysts and resulting chimeric males were bred to Tg(ACTFLPe)9205Dym/J females to remove the neo cassette. These mice express Cre-ER protein in pericytes in the vasculature of organs including brain and retina. Restricted to the cytoplasm, Cre-ER can only gain access to the nuclear compartment after exposure to tamoxifen. (J:263753)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pdgfrb (mouse)
Summary of all recombinase alleles driven by Pdgfrb.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pdgfrb Mutation:  87 strains or lines available
References
Original:  J:263753 Cuervo H, et al., PDGFRbeta-P2A-CreER(T2) mice: a genetic tool to target pericytes in angiogenesis. Angiogenesis. 2017 Nov;20(4):655-662
All:  27 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory