Npytm1.1(flpo)Hze
Targeted Allele Detail
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| Symbol: |
Npytm1.1(flpo)Hze |
| Name: |
neuropeptide Y; targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:5903980 |
| Synonyms: |
Npy-IRES2-FlpO |
| Gene: |
Npy Location: Chr6:49799690-49806487 bp, + strand Genetic Position: Chr6, 24.04 cM, cytoband B3
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| Alliance: |
Npytm1.1(flpo)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Npytm1.1(flpo)Hze expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Npy (MGI:97374) |
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Mutation details: The targeting vector contains, from 5' to 3', a partial Npy sequence spanning exon 4 (including the endogenous stop codon), an internal ribosome entry site 2 sequence, a FlpO recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL).
(J:260362)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Npy
(mouse)
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| Original: |
J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22 |
| All: |
17 reference(s) |
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Analysis Tools
