Esr2tm1.1(cre)Hze
Targeted Allele Detail
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| Symbol: |
Esr2tm1.1(cre)Hze |
| Name: |
estrogen receptor 2 (beta); targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:5904044 |
| Synonyms: |
Esr2-IRES2-Cre-D |
| Gene: |
Esr2 Location: Chr12:76167193-76224033 bp, - strand Genetic Position: Chr12, 33.52 cM, cytoband D1-D3
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| Alliance: |
Esr2tm1.1(cre)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Esr2tm1.1(cre)Hze expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a partial Esr2 sequence spanning exon 10 (including the endogenous stop codon), an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::FRT5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). The resulting Esr2-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice. Cre recombinase expression/activity largely recapitulates the endogenous Esr2 gene expression in the brain - observed mainly in medial amygdalar nucleus, bed nuclei of the stria terminalis, paraventricular hypothalamic nucleus, cortical layer 6 and scattered neurons elsewhere in the brain.
(J:146821)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Esr2
(mouse)
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| Original: |
J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06; |
| All: |
10 reference(s) |
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Analysis Tools
