Htr1atm1.1(cre)Hze
Targeted Allele Detail
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| Symbol: |
Htr1atm1.1(cre)Hze |
| Name: |
5-hydroxytryptamine (serotonin) receptor 1A; targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:5904051 |
| Synonyms: |
Htr1a-IRES2-Cre-D |
| Gene: |
Htr1a Location: Chr13:105580147-105584630 bp, + strand Genetic Position: Chr13, 56.92 cM
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| Alliance: |
Htr1atm1.1(cre)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Htr1atm1.1(cre)Hze expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Htr1a (MGI:96273) |
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Mutation details: The targeting vector contained, from 5' to 3', a partial Htr1a sequence spanning exon 1 (including the endogenous stop codon), an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). Cre recombinase expression/activity is observed mainly in cortex, hippocampus and retro-hippocamal regions, as well as scattered neurons elsewhere in the brain.
(J:146821)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Htr1a
(mouse)
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| Original: |
J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06; |
| All: |
5 reference(s) |
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Analysis Tools
