Pdyn<tm1.1(cre/ERT2)Hze> Targeted Allele Detail MGI Mouse (MGI:5904056)

Pdyn^{tm1.1(cre/ERT2)Hze}
Targeted Allele Detail

Summary

Symbol:	Pdyn^{tm1.1(cre/ERT2)Hze}
Name:	prodynorphin; targeted mutation 1.1, Hongkui Zeng
MGI ID:	MGI:5904056
Synonyms:	Pdyn-T2A-CreERT2-D
Gene:	Pdyn Location: Chr2:129528485-129541764 bp, - strand Genetic Position: Chr2, 63.19 cM
Alliance:	Pdyn^{tm1.1(cre/ERT2)Hze} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:146821
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Pdyn^{tm1.1(cre/ERT2)Hze} expression driven by 1 gene

Mutation details: The targeting vector contained, from 5' to 3', a partial Pdyn sequence spanning exon 4 (including the endogenous stop codon), a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL). The tamoxifen-inducible Cre recombinase activity is observed mainly in central amygdalar nucleus, paraventricular hypothalamic nucleus, nucleus accumbens, caudoputamen and cortex. (J:146821)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdyn (mouse) Summary of all recombinase alleles driven by Pdyn. Alliance mouse Pdyn gene page

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Pdyn Mutation:	20 strains or lines available

References

Original:	J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06;
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/19/2024 MGI 6.24