Gt(ROSA)26Sortm14.1(Mir150)Rsky
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm14.1(Mir150)Rsky |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 14.1, Klaus Rajewsky |
| MGI ID: |
MGI:5904751 |
| Synonyms: |
Rosa-26-flox-Stop-flox-miR-150fl, Stop-150fl |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm14.1(Mir150)Rsky page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:240474
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| Parent Cell Line: |
Other (see notes) (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Conditional ready, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Gt(ROSA)26Sortm14.1(Mir150)Rsky expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Mir150 (MGI:2676835) |
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Mutation details: The following elements were inserted into the locus: the CAG promoter and a loxP site flanked neomycin resistance gene and STOP cassette; genomic DNA including the microRNA stem loop with 136 bp of 5' and 130 bp of 3' flanking sequence; an frt site flanked IRES-EGFP cassette; and finally a polyadenylation signal. The EGFP cassette was deleted through subsequent FLP-mediated recombination. It is possible to generate allele Gt(ROSA)26Sortm2.1(Mirn150)Rsky from this allele.
(J:127234)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Gt(ROSA)26Sor Mutation: |
993 strains or lines available
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ES cell line = R19 (ARF1 R19 ES cells from Artemis Pharmaceuticals, Germany)
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| Original: |
J:127234 Xiao C, et al., MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007 Oct 5;131(1):146-59 |
| All: |
2 reference(s) |
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Analysis Tools
