Gene Expression Data
Assay Details
Assay
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Reference:
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J:31368
Reya T, et al., Regulated expression and function of CD122 (interleukin-2/interleukin-15R-beta) during lymphoid development. Blood. 1996 Jan 1;87(1):190-201
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5906400
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Gene symbol:
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Il2rb
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Gene name:
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interleukin 2 receptor, beta chain
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Probe:
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CD122 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: hemolymphoid system |
Present |
Regionally restricted |
3A
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High levels of expression in individual cells in close proximity to vascular endothelium of branchial blood vessel directly above developing pharynx.
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TS20: craniocervical region |
Present |
Regionally restricted |
3A
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Expression within connective tissue immediately below developing pharynx.
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Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: craniocervical region |
Strong |
Regionally restricted |
3B
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Distinct clusters of expressing cells in association with connective tissue below the pharynx.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: thymus primordium |
Present |
Regionally restricted |
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Expression in cells localized to base of stem of thymic anlagen in region of third pharyngeal pouch.
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: craniocervical region |
Present |
Regionally restricted |
3C
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Authors report a similar distribution of expressing cells in additional sections processed in parallel or separately.
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