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Gene Expression Data
Assay Details
Assay
Reference: J:240500 Lopez-Domenech G, et al., Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse Model with Disrupted Mitochondrial Distribution in Mature Dendrites. Cell Rep. 2016 Oct 04;17(2):317-327
Assay type: Western blot
MGI Accession ID: MGI:5906563
Gene symbol: Rhot1
Gene name: ras homolog family member T1
Antibody: anti-Miro1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Antibody recognizes both Miro1 (Rhot1; duplex at 71-73 kDa) and Miro2 (Rhot2; 74 kDa). Both genes have been annotated.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 71 kDa (b) Genetic BackgroundMutant Allele(s)Sex
Miro1 WT E18.0 (a) TS26: brain Present involves: C57BL/6Dnk * C57BL/6N Not Specified
Miro1 WT E18.0 (a) TS26: brain Present involves: C57BL/6Dnk * C57BL/6N Not Specified
Miro1 HET E18.0 (a) TS26: brain Present involves: C57BL/6Dnk * C57BL/6N Rhot1tm1d(EUCOMM)Wtsi/Rhot1+Not Specified
Miro1 HET E18.0 (a) TS26: brain Present involves: C57BL/6Dnk * C57BL/6N Rhot1tm1d(EUCOMM)Wtsi/Rhot1+Not Specified
Miro1 KO E18.0 (a) TS26: brain Absent (c) involves: C57BL/6Dnk * C57BL/6N Rhot1tm1d(EUCOMM)Wtsi/Rhot1tm1d(EUCOMM)WtsiNot Specified
Miro1 KO E18.0 (a) TS26: brain Absent (c) involves: C57BL/6Dnk * C57BL/6N Rhot1tm1d(EUCOMM)Wtsi/Rhot1tm1d(EUCOMM)WtsiNot Specified
Miro2 HET E18.0 (a) TS26: brain Present involves: C57BL/6N * C57BL/6NTac Rhot2tm1(KOMP)Wtsi/Rhot2+Not Specified
Miro2 KO E18.0 (a) TS26: brain Present involves: C57BL/6N * C57BL/6NTac Rhot2tm1(KOMP)Wtsi/Rhot2tm1(KOMP)WtsiNot Specified
Miro2 WT E18.0 (a) TS26: brain Present involves: C57BL/6N * C57BL/6NTac Not Specified
Miro2 WT E18.0 (a) TS26: brain Present involves: C57BL/6N * C57BL/6NTac Not Specified
Miro2 HET E18.0 (a) TS26: brain Present involves: C57BL/6N * C57BL/6NTac Rhot2tm1(KOMP)Wtsi/Rhot2+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Duplex at 71-73 kDa
(c) Authors report specific deletion of proteins in knock out animals.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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