Probe:	very-KIND probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: forebrain	Weak		3A
TS22: hindbrain	Present		3A	Expression mainly in midbrain, hindbrain and neural tube.
TS22: midbrain	Present		3A	Expression mainly in midbrain, hindbrain and neural tube.
TS22: spinal cord	Present		3A	Expression mainly in midbrain, hindbrain and neural tube.

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Strong		3B	Strong expression shifts toward telencephalon.
TS25: spinal cord	Present		3B	Expression in neural tube persisted at E17.5.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3C	Expression in neural tube started at ventral-lateral edge.
TS17: dorsal root ganglion	Absent		3C

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3F	Expression in neural tube expanded dorsally during development.
TS20: dorsal root ganglion	Present	Regionally restricted	3F	Expression in a few cells in the dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3I	Speckled pattern of expression in entire neural tube, except for marginal layer.
TS22: spinal cord marginal layer	Absent		3I

Structure	Level	Pattern	Image	Note
TS25: neural retina	Present	Regionally restricted	4A	Expression predominantly in future neuronal cell layers. Authors report specific expression in retinal ganglion cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		5A	Expression most prominent in cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5B	Expression restricted to granular and Purkinje cells layers of cerebellum.
TS28: cerebellum granule cell layer	Present		5B	Expression restricted to granular and Purkinje cells layers of cerebellum.
TS28: cerebellum molecular layer	Absent		5B	No expression in molecular layer or branching white matter of cerebellum.
TS28: cerebellum white matter	Absent		5B	No expression in molecular layer or branching white matter of cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5C	Expression in large cells, most likely in neurons, throughout most of the cerebral cortex.