Tg(CMV-Il33)50Lizh
Transgene Detail
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| Symbol: |
Tg(CMV-Il33)50Lizh |
| Name: |
transgene insertion 50, Lianfeng Zhang |
| MGI ID: |
MGI:5910699 |
| Transgene: |
Tg(CMV-Il33)50Lizh Location: unknown
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| Alliance: |
Tg(CMV-Il33)50Lizh page
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| Transgene Type: |
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Transgenic (Dominant negative, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(CMV-Il33)50Lizh expresses
1 gene
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Mutation details: The full-length mouse cDNA was cloned into the pCDNA3.1(+) vector downstream of the CMV promoter and upstream of the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence. Linearized vector was injected into the pronucleus of fertilized C57BL/6J zygotes. Five transgenic founders (number #14, #50, #55, #63 and #79) were established. The transgene copy number in F1, F2 and F3 generations of founder #50 was determined to be around 17 on average. Western blot analysis showed the expression of the 18 kDa product from the transgene.
(J:243722)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:243722 Zhiguang X, et al., Over-expression of IL-33 leads to spontaneous pulmonary inflammation in mIL-33 transgenic mice. Immunol Lett. 2010 Jul 08;131(2):159-65 |
| All: |
6 reference(s) |
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Analysis Tools
