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Fostm2.1(icre/ERT2)Luo
Targeted Allele Detail
Summary
Symbol: Fostm2.1(icre/ERT2)Luo
Name: FBJ osteosarcoma oncogene; targeted mutation 2.1, Liqun Luo
MGI ID: MGI:5910950
Synonyms: Fos2A-iCreER, Fos2A-iCreERT2, TRAP2
Gene: Fos  Location: Chr12:85520664-85524047 bp, + strand  Genetic Position: Chr12, 39.7 cM
Alliance: Fostm2.1(icre/ERT2)Luo page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Fostm2.1(icre/ERT2)Luo expression driven by 1 gene
 
Mutation details: A targeting vector was designed to insert a 2A::iCreER::frt-neo-frt cassette into the translational stop site of the FBJ osteosarcoma oncogene locus (Fos) on chromosome 12. The viral 2A oligopeptide sequence mediates ribosomal skipping. The iCreER fusion gene used here has an improved/optimized variant of Cre recombinase (iCre) that is fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain (ER). TThe targeting construct was electroporated into 129;FVB-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred to CD1 mice and/or germline-active GFP-FlpO transgenic mice (mixed genetic background including CD1) to delete the frt-flanked neo cassette. (J:101977, J:250613)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Fos (mouse)
Summary of all recombinase alleles driven by Fos.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fos Mutation:  43 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  73 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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11/12/2024
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