Gt(ROSA)26Sortm12.1Mgn
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm12.1Mgn |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 12.1, Mark A Magnusson |
| MGI ID: |
MGI:5911670 |
| Synonyms: |
Gt(ROSA)26Sortm1(Pdx1,MafA,Neurog3.mCherry)Mgn, Rosa263TF.mCherry, Rosa26(3TF.mCherry)Mgn, Rs263TF.mCherry |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm12.1Mgn page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:241149
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| Parent Cell Line: |
TL1/TL-1 (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted (Inducible, Inserted expressed sequence, RMCE-ready, Reporter) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Gt(ROSA)26Sortm12.1Mgn expresses
3 genes
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Mutation details: In mice bearing this allele, doxycycline regulates expression of the mCherry fluorescent reporter and three mouse transcription factors (3TF) - v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (encoded in mouse by Mafa), pancreatic and duodenal homeobox 1 (Pdx1), and neurogenin 3 (Neurog3) - in cells expressing either the tetracycline transactivator (tTA) or reverse transactivator (rtTA). The allele was generated by recombinase-mediated cassette exchange (RMCE) in ES cells harboring the cassette acceptor allele Gt(ROSA)26Sortm1Mgn. The insertion, flanked by lox71/lox2272 sites, contains a bi-directional tetracycline regulated promoter (a tetracycline responsive element (TRE), comprising seven copies of the tetracycline operator (tetO), flanked by CMV mini promoters) derived from pTRE-Tight-BI driving expression, in one direction, of mCherry, and in the opposite direction, of a single transcript encoding the 3TF separated by 2A peptides, which mediate ribosome skipping via inhibition of peptide bond formation. Excision of a selection cassette has left a single FRT site downstream of mCherry.
(J:241149)
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| Original: |
J:241149 Clayton HW, et al., Pancreatic Inflammation Redirects Acinar to beta Cell Reprogramming. Cell Rep. 2016 Nov 15;17(8):2028-2041 |
| All: |
1 reference(s) |
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