Slc22a6tm1.1(cre/ERT2)Amat
Targeted Allele Detail
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| Symbol: |
Slc22a6tm1.1(cre/ERT2)Amat |
| Name: |
solute carrier family 22 (organic anion transporter), member 6; targeted mutation 1.1, Athena Matakidou |
| MGI ID: |
MGI:5911847 |
| Synonyms: |
Slc22a6-CreERT2 |
| Gene: |
Slc22a6 Location: Chr19:8595403-8605663 bp, + strand Genetic Position: Chr19, 5.44 cM
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| Alliance: |
Slc22a6tm1.1(cre/ERT2)Amat page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:243751
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| Parent Cell Line: |
Other (see notes) (ES Cell)
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| Strain of Origin: |
C57BL/6J
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutations: |
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Insertion, Intragenic deletion
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Slc22a6tm1.1(cre/ERT2)Amat expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Slc22a6 (MGI:892001) |
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Mutation details: Using recombineering methods, the CreERT2-Frt-PGK-Hygromycin-pA-Frt cassette replaced the ATG start codon in exon 1 of the Slc22a6 gene in the BAC clone RP23-457i11. The modified BAC construct was electroporated into ES cells and correctly targeted ES cells were injected into CD1 blastocysts and the resulting chimeric mice were bred to C57BL/6J mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked hygromycin selection cassette.
(J:243751)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Slc22a6
(mouse)
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C57BL/6J embryonic stem cells (CRIB6.1, an ES line derived in house) were used.
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| Original: |
J:243751 Espana-Agusti J, et al., Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells. PLoS One. 2016;11(2):e0148055 |
| All: |
1 reference(s) |
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Analysis Tools
