Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:18773
Ganju P, et al., The Eck receptor tyrosine kinase is implicated in pattern formation during gastrulation, hindbrain segmentation and limb development. Oncogene. 1994 Jun;9(6):1613-24
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Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
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MGI:5925302
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Gene symbol:
|
Epha2
|
Gene name:
|
Eph receptor A2
|
Antibody:
|
anti-Eck
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Results
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Specimen
E6.5 not shown:
embryonic day 6.5
(more )
Specimen
E6.5 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 6.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: primitive streak |
Weak |
|
|
Low level of expression specifically in cells of streak.
|
Specimen
3A:
embryonic day 7.5
(more )
Note: Authors state that embryo is at late streak stage.
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 7.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Note:
|
Authors state that embryo is at late streak stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: primitive streak |
Present |
|
3A
|
Authors report expression in region of primitive streak.
|
TS10: embryo |
Strong |
Regionally restricted |
3A
|
Strong expression in midline.
|
Specimen
3B:
embryonic day 7.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 7.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: notochordal plate |
Strong |
|
3B
|
Strong expression in notochordal plate relative to lateral mesoderm.
|
TS11: embryo ectoderm |
Present |
Regionally restricted |
3B
|
Expression in ectodermal cells in midline immediately overlying notochordal plate, relative to more lateral regions of ectoderm.
|
Specimen
3C:
embryonic day 9.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: notochordal plate |
Strong |
|
3C
|
High levels of expression restricted to progressively more caudal regions containing notochordal plate cells.
|
TS14: future spinal cord neural plate |
Present |
|
3C
|
Expression in neural plate cells in caudal regions.
|
TS14: dorsal aorta |
Present |
Regionally restricted |
3C
|
Uniform distribution of expression in cells lining the dorsal aorta.
|
TS14: gut |
Weak |
Regionally restricted |
3C
|
Low level of expression in caudal regions of hindgut.
|
Specimen
3D:
embryonic day 9.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: notochord |
Weak |
|
3D
|
|
TS15: dorsal aorta |
Present |
Regionally restricted |
3D
|
Uniform distribution of expression in cells lining the dorsal aorta.
|
TS15: migrating neural crest |
Strong |
Regionally restricted |
3D
|
High levels of expression in presumptive neural crest cells aggregating as dorsal root ganglia between somites and neural tube in the trunk.
|
Specimen
4A:
embryonic day 8.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future hindbrain |
Present |
Regionally restricted |
4A
|
Single lateral band of expression in hindbrain neuroepithelia. Limits to this expression seen cranially at level of preotic sulcus.
|
TS13: 2nd branchial arch |
Present |
|
4A
|
Expression extended ventrolaterally in cells occupying the second branchial arch.
|
TS13: trophoblast giant cell |
Present |
|
4A
|
|
Specimen
4B:
embryonic day 8.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future hindbrain |
Present |
Regionally restricted |
4B
|
Single lateral band of expression in hindbrain neuroepithelia. Limits to this expression seen cranially at level of preotic sulcus.
|
TS13: 2nd branchial arch |
Present |
|
4B
|
Expression extended ventrolaterally in cells occupying the second branchial arch.
|
TS13: trophoblast giant cell |
Present |
|
4B
|
|
Specimen
4C:
embryonic day 8.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: 2nd branchial arch |
Present |
|
4C
|
Expression extended ventrolaterally in cells occupying the second branchial arch.
|
Specimen
4D:
embryonic day 8.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: 2nd branchial arch |
Present |
|
4D
|
Expression extended ventrolaterally in cells occupying the second branchial arch
|
TS13: future hindbrain |
Present |
Regionally restricted |
4D
|
Limits to this expression seen caudally at level of otic placode.
|
Specimen
4E:
embryonic day 8.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future hindbrain |
Present |
Regionally restricted |
4E
|
Limits to this expression seen caudally at level of otic placode.
|
Specimen
4F:
embryonic day 8.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
5B:
embryonic day 9.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
E9.5 not shown:
embryonic day 9.5
(more )
Specimen
E9.5 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
5D:
embryonic day 10.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
E10.5 not shown:
embryonic day 10.5
(more )
Specimen
E10.5 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: 3rd branchial arch |
Weak |
|
|
Little expression detectable in cells of third branchial arches or facial ganglia at E10.5.
|
TS17: lung |
Present |
Regionally restricted |
|
Expression in epitheium of segmented bronchi as early as E10.5.
|
TS17: infundibular recess of 3rd ventricle |
Present |
|
|
Expression in infundibular recess emanating from floor of diencephalon as early at E10.5.
|
Specimen
6A:
embryonic day 10.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
6A
|
Expression first detected in mesenchyme of forelimb bud at E10.5, localized specifically to distal mesenchyme.
|
Specimen
6B:
embryonic day 10.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
6B
|
Expression first detected in mesenchyme of hindlimb buds at E10.5, localized specifically to distal mesenchyme.
|
Specimen
6C:
embryonic day 11.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate |
Present |
|
6C
|
Differential expression along the proximal-distal axis persisted at E11.5. Expression localized to hanplate region.
|
Specimen
6D:
embryonic day 11.5
(more )
Specimen
6D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: footplate |
Present |
|
6D
|
Differential expression along the proximal-distal axis persisted at E11.5. Expression localized to footplate region.
|
Specimen
6E:
embryonic day 14.5
(more )
Specimen
6E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: humerus cartilage condensation |
Present |
Regionally restricted |
6E
|
Expression in perichondria and chondrocytes of developing long bones regardless of their position along the proximal-distal axis.
|
TS22: radius cartilage condensation |
Present |
Regionally restricted |
6E
|
Expression in perichondria and chondrocytes of developing long bones regardless of their position along the proximal-distal axis.
|
Specimen
6F:
embryonic day 15.5
(more )
Specimen
6F:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: carpal bone |
Present |
Regionally restricted |
6F
|
Expression in perichondria and chondrocytes of developing long bones regardless of their position along the proximal-distal axis.
|
TS23: forelimb phalanx |
Present |
Regionally restricted |
6F
|
Expression in perichondria and chondrocytes of developing long bones regardless of their position along the proximal-distal axis.
|
TS23: metacarpal bone |
Present |
Regionally restricted |
6F
|
Expression in perichondria and chondrocytes of developing long bones regardless of their position along the proximal-distal axis.
|
TS23: ulna |
Present |
Regionally restricted |
6F
|
Expression in perichondria and chondrocytes of developing long bones regardless of their position along the proximal-distal axis.
|
TS23: forelimb epidermis |
Present |
|
6F
|
Expression in epithelia of limb.
|
Specimen
7A:
embryonic day 14.5
(more )
Specimen
7A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
7B:
embryonic day 14.5
(more )
Specimen
7B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
7C:
embryonic day 14.5
(more )
Specimen
7C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: rib cartilage condensation |
Present |
Regionally restricted |
7C
|
Expression in perichondria and chondrocytes of developing ribs.
|
TS22: lung |
Present |
Regionally restricted |
7C
|
Expression in epithelia of terminal bronchi and bronchioles.
|
Specimen
7D:
embryonic day 14.5
(more )
Specimen
7D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
7E:
embryonic day 14.5
(more )
Specimen
7E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
7F:
embryonic day 12.5
(more )
Specimen
7F:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
|