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Chia1tm1.1(icre)Lky
Targeted Allele Detail
Summary
Symbol: Chia1tm1.1(icre)Lky
Name: chitinase, acidic 1; targeted mutation 1.1, Richard M Locksley
MGI ID: MGI:5927647
Synonyms: Chia1tm1.1(icre,tdTomato)Lky, ChiaRed
Gene: Chia1  Location: Chr3:106020698-106039434 bp, + strand  Genetic Position: Chr3, 46.49 cM, cytoband F3
Alliance: Chia1tm1.1(icre)Lky page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:241504
Parent Cell Line:  PRX-B6T (ES Cell)
Strain of Origin:  C57BL/6J
Mutation
description
Allele Type:    Targeted (Null/knockout, Recombinase, Reporter)
Mutation:    Insertion
 
Chia1tm1.1(icre)Lky expression driven by 1 gene
 
Mutation details: A composite fluorescent reporter/recombinase cassette was introduced to exon 1 of the mouse Chia1 gene via homologous recombination embryonic stem (ES) cells. The targeting vector incorporated rabbit beta-globin (exon 2-3 partial genomic sequence serving as an artificial intron and splicing donor/acceptor), tandem tomato (tdTomato) fluorescent reporter, encephalomyocarditic virus (EMCV) internal ribosome entry site (IRES), P1 phage cre recombinase cDNA optimized for mammalian cells (hCre; iCre), bovine growth hormone (bGH) poly-adenylation signal (pA), and a floxed neomycin resistance cassette. The loxP-flanked neomycin cassette was excised from the resultant mice via crosses with B6.C-Tg(CMV-cre)1Cgn/J animal. (J:241504)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Chia1 (mouse)
Summary of all recombinase alleles driven by Chia1.
 

Expression
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chia1 Mutation:  53 strains or lines available
References
Original:  J:241504 Van Dyken SJ, et al., Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease. Cell. 2017 Apr 20;169(3):497-509.e13
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory