Probe:	Y2 probe mix
Probe preparation:	Antisense labelled with S35 DNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Absent		2_Y2 top row

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Absent		2_Y2 2nd row

Structure	Level	Pattern	Image	Note
TS28: olfactory tubercle	Present	Regionally restricted	2_Y2 3rd row	No expression in olfactory tubercle grooves.
TS28: islands of Calleja	Absent		2_Y2 3rd row
TS28: piriform cortex	Absent		2_Y2 3rd row
TS28: basal ganglia	Present	Regionally restricted	2_Y2 3rd row
TS28: globus pallidus	Present	Scattered	2_Y2 3rd row	Scattered positive cells in globus pallidus.
TS28: corpus striatum	Present	Single cells	2_Y2 3rd row	Few positive cells in the striatum.
TS28: accumbens nucleus	Weak		2_Y2 3rd row	Low expression in many cells in nucleus accumbens.
TS28: telencephalon septum	Present	Regionally restricted	2_Y2 3rd row	Expression in septal area extending into ventral pallidum

Structure	Level	Pattern	Image	Note
TS28: CA3 pyramidal cell layer	Strong		2_Y2 4th row
TS28: dentate gyrus granule cell layer	Present		2_Y2 4th row
TS28: CA1 pyramidal cell layer	Weak		2_Y2 4th row
TS28: neocortex	Absent		2_Y2 4th row
TS28: anterior amygdaloid area	Present		2_Y2 4th row
TS28: arcuate nucleus	Present	Regionally restricted	2_Y2 4th row

Structure	Level	Pattern	Image	Note
TS28: dorsal motor nucleus of vagus nerve	Present		2_Y2 bottom row

Structure	Level	Pattern	Image	Note
TS28: CA3 pyramidal cell layer	Present		4A
TS28: dentate gyrus granule cell layer	Present		4A
TS28: anterior amygdaloid area	Strong	Regionally restricted	4A	Abundant expression in amygdaloid area.
TS28: arcuate nucleus	Present	Regionally restricted	4A

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		4B

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		4C
TS28: hippocampus CA3	Present		4C
TS28: arcuate nucleus	Present	Regionally restricted	4C	Expression in ventromedial arcuate nucleus.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted		No expression in cortex-amygdala transition zone
TS28: olfactory tract	Absent			No expression in lateral olfactory tract.
TS28: cingulate cortex	Absent
TS28: entorhinal cortex	Absent
TS28: presubiculum	Absent
TS28: subiculum	Absent
TS28: amygdala	Present	Regionally restricted		Expression in cells scattered in both basal and medial areas of amygdala.
TS28: basolateral amygdaloid nucleus	Present
TS28: central amygdaloid nucleus	Present
TS28: basal forebrain	Present	Regionally restricted
TS28: bed nucleus of stria terminalis	Present
TS28: hypothalamus	Weak	Regionally restricted		Authors report a similar pattern of expression as seen for Y1 (see assay MGI:6107952) but with much few positive cells. Expression in cells scattered throughout the lateral hypothalamus.
TS28: hypothalamus periventricular zone	Present			Expression in subparaventricular zone, periventricular zone, paraventricular hypothalamus and medial parvicellular area.
TS28: hypothalamus subparaventricular zone	Present			Expression in subparaventricular zone, periventricular zone, paraventricular hypothalamus and medial parvicellular area.
TS28: paraventricular hypothalamic nucleus	Present			Expression in subparaventricular zone, periventricular zone, paraventricular hypothalamus and medial parvicellular area.
TS28: medial preoptic area	Absent			No expression in suprachiasmatic nucleus, medial preoptic area or dorsal premammillary nucleus.
TS28: suprachiasmatic nucleus	Absent			No expression in suprachiasmatic nucleus, medial preoptic area or dorsal premammillary nucleus.
TS28: area postrema	Present
TS28: claustrum	Present
TS28: dorsal raphe nucleus	Present

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present	Regionally restricted	5B	Expression in less than 5% of neurons; these neurons belong to the small and medium size population.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present	Regionally restricted	5D	Expression in less than 5% of neurons; these neurons belong to the small and medium size population.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Weak	Regionally restricted	5F	Less cells appear to be Y2-positive than Y1-positive (see assay MGI:6107952). Y2-expression mostly in the medium and large size population of neurons.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Weak	Regionally restricted	5H	Less cells appear to be Y2-positive than Y1-positive (see assay MGI:6107952). Y2-expression mostly in the medium and large size population of neurons.

Structure	Level	Pattern	Image	Note
TS28: inferior vagus ganglion	Strong	Regionally restricted	5I	Abundant expression in almost half of nodose-petrosal neurons, predominantly localized to the nodose ganglion.

Structure	Level	Pattern	Image	Note
TS28: inferior glossopharyngeal ganglion	Present	Regionally restricted	5J	Expression in a small proportion of petrosal ganglion neurons.

Structure	Level	Pattern	Image	Note
TS28: superior cervical ganglion	Absent