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Penktm1.1(cre)Gsche
Targeted Allele Detail
Summary
Symbol: Penktm1.1(cre)Gsche
Name: preproenkephalin; targeted mutation 1.1, Gregory Scherrer
MGI ID: MGI:6113637
Synonyms: Penkcre
Gene: Penk  Location: Chr4:4133536-4138815 bp, - strand  Genetic Position: Chr4, 2.31 cM
Alliance: Penktm1.1(cre)Gsche page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:253245
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEvTac or C57BL/6J
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Penktm1.1(cre)Gsche expression driven by 1 gene
 
Mutation details: The targeting vector was constructed using recombineering techniques. An 8,528 bp genomic DNA fragment containing the last exon of the mouse Penk gene was retrieved from BAC clone RP23-RP23- 365K8 to a vector containing the DT gene. A cassette of IRES-Cre frt-PGKNeo-frt was inserted between stop codon TGA and 3' UTR. The neo cassette was removed by breeding germline chimeras with FLP1 expressing homozygous females. (J:253245)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Penk (mouse)
Summary of all recombinase alleles driven by Penk.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Penk Mutation:  22 strains or lines available
Notes
"The targeting vector was electroporated into F1 hybrid of 129S6 x C57BL/6J ES cells derived by Janelia Transgenic Facility." (J:253245)
References
Original:  J:253245 Francois A, et al., A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins. Neuron. 2017 Feb 22;93(4):822-839.e6
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory