Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:251844
Zamboni V, et al., Disruption of ArhGAP15 results in hyperactive Rac1, affects the architecture and function of hippocampal inhibitory neurons and causes cognitive deficits. Sci Rep. 2016 Oct 7;6:34877
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6115354
|
|
Gene symbol:
|
Slc17a7
|
|
Gene name:
|
solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
|
|
Antibody:
|
anti-VGLUT
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 568
|
|
|
Results
|
Specimen
4a Wild-type:
postnatal day 30
(more  )
Specimen
4a Wild-type:
(close  )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus CA1 |
Present |
Regionally restricted |
4a Wild-type
|
Expression in puncta on perisomatic surface of pyramidal neurons (projections from CA3 pyramidal neurons.
|
| TS28: hippocampus CA2 |
Present |
Regionally restricted |
4a Wild-type
|
Expression in puncta on perisomatic surface of pyramidal neurons (projections from CA3 pyramidal neurons.
|
Specimen
4a ArhGAP45-/-:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * DBA
|
|
Mutant Allele(s):
|
Arhgap15tm1Ehi/Arhgap15tm1Ehi
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus CA1 |
Present |
Regionally restricted |
4a ArhGAP15-/-
|
Expression in puncta on perisomatic surface of pyramidal neurons (projections from CA3 pyramidal neurons.
|
| TS28: hippocampus CA2 |
Present |
Regionally restricted |
4a ArhGAP15-/-
|
Expression in puncta on perisomatic surface of pyramidal neurons (projections from CA3 pyramidal neurons.
|
Specimen
4b Wild-type:
postnatal day 30
(more )
Specimen
4b Wild-type:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4b ArhGAP15-/-:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * DBA
|
|
Mutant Allele(s):
|
Arhgap15tm1Ehi/Arhgap15tm1Ehi
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
not shown Wild-type:
postnatal day 30
(more )
Specimen
not shown Wild-type:
(close )
|
Genetic Background:
|
involves: C57BL/6 * DBA
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dentate gyrus |
Present |
Regionally restricted |
|
Expression in projections from the entorhinal cortex.
|
Specimen
not shown ArhGAP15-/-:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * DBA
|
|
Mutant Allele(s):
|
Arhgap15tm1Ehi/Arhgap15tm1Ehi
|
|
Age:
|
postnatal day 30
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dentate gyrus |
Present |
Regionally restricted |
|
No difference detected between wild-type and mutants.
|
|
Analysis Tools