Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106304
Jeays-Ward K, et al., Wnt4 is required for proper male as well as female sexual development. Dev Biol. 2004 Dec 15;276(2):431-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6117303
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Gene symbol:
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Cyp11a1
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Gene name:
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cytochrome P450, family 11, subfamily a, polypeptide 1
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Probe:
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Cyp11a1 probe
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Assay notes:
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Authors refer to specimens that are not homozygous Wnt4-/- as wild-type, but report they can be either +/+ or +/-, and report no differences were found between +/+ and Wnt+/- embryos for all genes analyzed at all stages.
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Results
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Specimen
3Aa:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 10.0-10.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: gonad primordium |
Present |
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3Aa
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Authors report a significant increase in the number of expressing cells in mutant gonads compared with wild-type.
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Specimen
3Ab:
embryonic day 10.0-10.5
(more  )
Specimen
3Ab:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 10.0-10.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3A XX -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 11.5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
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3A XX -/-
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Authors report a significant increase in the number of expressing cells in mutant gonads compared with wild-type.
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| TS19: endocrine gland |
Present |
Regionally restricted |
3A XX -/-
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Authors report expression in adrenal glands.
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Specimen
3A XX (right pane):
embryonic day 11.5
(more )
Specimen
3A XX (right pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 11.5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3A XY -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
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3A XY -/-
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Authors report a significant increase in the number of expressing cells in mutant gonads compared with wild-type.
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| TS19: endocrine gland |
Present |
Regionally restricted |
3A XY -/-
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Authors report expression in adrenal glands.
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Specimen
3A XY (right pane):
embryonic day 11.5
(more )
Specimen
3A XY (right pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3B -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
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3B -/-
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Authors report a significant increase in the number of expressing cells in mutant gonads compared with wild-type.
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Specimen
3B (right pane):
embryonic day 11.5
(more )
Specimen
3B (right pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4A -/- (center pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
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4A -/- (center pane)
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Authors report a significant increase in the number of expressing cells in mutant gonads compared with wild-type.
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Specimen
4A +/-:
embryonic day 11.5; Wnt4tm1Amc/Wnt4 +
(more )
Specimen
4A +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4+
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5e:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: testis |
Present |
Regionally restricted |
5e
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Authors report expression in fewer Leydig cells and that pattern of expression in Leydig cells was disorganized.
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Specimen
5f:
embryonic day 13.5
(more )
Specimen
5f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5g:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: testis |
Present |
Regionally restricted |
5g
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Authors report expression in fewer Leydig cells and that pattern of expression in Leydig cells was disorganized.
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Specimen
5h:
embryonic day 14.5
(more )
Specimen
5h:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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