Agtr1aem1(cre)Zak
Endonuclease-mediated Allele Detail
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| Symbol: |
Agtr1aem1(cre)Zak |
| Name: |
angiotensin II receptor, type 1a; endonuclease-mediated mutation 1, Zachary Knight |
| MGI ID: |
MGI:6117421 |
| Synonyms: |
Agtr1a-2a-Cre |
| Gene: |
Agtr1a Location: Chr13:30520424-30566850 bp, + strand Genetic Position: Chr13, 13.19 cM
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| Alliance: |
Agtr1aem1(cre)Zak page
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| Allele Type: |
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Endonuclease-mediated (Recombinase) |
| Mutation: |
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Insertion
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Agtr1aem1(cre)Zak expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Agtr1a (MGI:87964) |
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Mutation details: The bicistronic Agtr1a-2A-Cre knock-in allele was created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (cre) immediately upstream of the endogenous STOP codon of Agtr1a gene. The targeting vector, Cas9 endonuclease and single guide RNA were mixed and injected into the pronuclei of FVB/N fertilized zygotes. Embryos were transferred to pseudopregnant females, and correctly targeted pups were maintained on mixed FVB/C57BL/6J background. Two independent knock-in lines were crossed to reporter mice, and reporter expression patterns from these lines were identical.
(J:256118)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Agtr1a
(mouse)
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| Original: |
J:256118 Leib DE, et al., The Forebrain Thirst Circuit Drives Drinking through Negative Reinforcement. Neuron. 2017 Dec 20;96(6):1272-1281.e4 |
| All: |
3 reference(s) |
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Analysis Tools
