Tcrdtm1.1(cre/ERT2)Zhu
Targeted Allele Detail
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| Symbol: |
Tcrdtm1.1(cre/ERT2)Zhu |
| Name: |
T cell receptor delta chain; targeted mutation 1.1, Yuan Zhuang |
| MGI ID: |
MGI:6117766 |
| Synonyms: |
TCRdCreER |
| Gene: |
Tcrd Location: Chr14:54183530-54396655 bp, + strand Genetic Position: Chr14, Syntenic
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| Germline Transmission: |
Earliest citation of germline transmission:
J:257202
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
129S/SvEv
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tcrdtm1.1(cre/ERT2)Zhu expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Tcrd (MGI:98611) |
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Mutation details: A targeting construct was designed to insert an internal ribosome entry site (IRES) and a creERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand-binding domain) followed by a frt-flanked neomycin resistance (neo) cassette, just downstream of the coding region of the T cell receptor delta chain (Tcrd) gene. This construct was electroporated into 129/sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring were bred to C57BL/6-Tg(CAG-Flpe)2Arte mice to remove the neo cassette. Progeny was crossed to remove the Flp-expressing transgene and the resulting mice were bred to C57BL/6J inbred mice for at least 10 generations.
(J:257202)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tcrd
(mouse)
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| Original: |
J:257202 Zhang B, et al., Differential Requirements of TCR Signaling in Homeostatic Maintenance and Function of Dendritic Epidermal T Cells. J Immunol. 2015 Nov 1;195(9):4282-91 |
| All: |
17 reference(s) |
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Analysis Tools
