Mex3atm1.1(cre/ERT2)Ebat
Targeted Allele Detail
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| Symbol: |
Mex3atm1.1(cre/ERT2)Ebat |
| Name: |
mex3 RNA binding family member A; targeted mutation 1.1, Eduard Batlle |
| MGI ID: |
MGI:6121437 |
| Synonyms: |
Mex3atm1(tdTomato-T2A-CreERT2)EBa |
| Gene: |
Mex3a Location: Chr3:88439702-88448703 bp, + strand Genetic Position: Chr3, 38.86 cM
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| Alliance: |
Mex3atm1.1(cre/ERT2)Ebat page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:253826
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| Parent Cell Line: |
W4 (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted (Recombinase, Reporter) |
| Mutation: |
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Insertion
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Mex3atm1.1(cre/ERT2)Ebat expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A cassette bearing a tdTomato/T2A/Cre-ERT2/bGH polyA was inserted in frame with the mouse Mex3a start codon in exon 1. After homologous recomibination in ES cells, positive clones were expanded and then transfected with an FlpO-bearing plasmid to remove the neomycin cassette. Positive clones were selected and blastocyst injection was done to generate chimeras. The chimeras with successful germline transmission were bred with C57BL6 mice and the offspring was used to generate the final mouse colonies.
(J:253826)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Mex3a
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Mex3a Mutation: |
27 strains or lines available
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| Original: |
J:253826 Barriga FM, et al., Mex3a Marks a Slowly Dividing Subpopulation of Lgr5+ Intestinal Stem Cells. Cell Stem Cell. 2017 Jun 1;20(6):801-816.e7 |
| All: |
1 reference(s) |
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Analysis Tools
