Results
|
Specimen
1A,B,C:
embryonic day 10.5
(more )
Specimen
1A,B,C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: dorsal root ganglion |
Present |
|
1A
|
|
TS17: future spinal cord |
Present |
Regionally restricted |
1C
,
1A
,
1B
|
Expression was detected in motor neurons in the motor column and by their axons in the motor root. Immunopositive sensory axons were noted entering the dorsal horn. A small number of commissural axons were seen passing along the inside border of the motor column and then across the midline floor plate.
|
Specimen
1D,E,F:
embryonic day 17.5
(more )
Specimen
1D,E,F:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: spinal cord |
Present |
Regionally restricted |
1F
,
1E
,
1D
|
Expression was detected in the ascending and descending longitudinal pathways that consititute the dorsal columns, dorsal funiculus, and ventrolateral funiculus. There was no expression in the motor column, spinal motor axons, the dorsal root entry zone, or commissural axons as they coursed towards the midline and crossed the floor plate.
|
TS25: dorsal root ganglion |
Absent |
|
1D
|
|
Specimen
2A:
embryonic day 14.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2B,E,H:
embryonic day 17.5
(more )
Note: Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
Specimen
2B,E,H:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
|
Specimen
2C:
postnatal day 0
(more )
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2D,I,L:
postnatal day 10
(more )
Note: Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
Specimen
2D,I,L:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: main olfactory bulb olfactory nerve layer |
Strong |
|
2I
,
2L
,
2D
|
|
TS28: main olfactory bulb layer |
Strong |
Regionally restricted |
2L
,
2I
,
2D
|
Strong expression was detected throughout the outer layers of the olfactory bulb, which include the olfactory sensory axons (as indicated by co-expression with Gap43).
|
TS28: main olfactory bulb glomerular layer |
Weak |
Regionally restricted |
2L
,
2D
,
2I
|
Expression was detected exclusively in the pre-terminal portions of olfactory sensory axons within the nerve fiber layer and as the axons enter the glomerular layer, but it is not present in the glomerular neuropil containing the terminal portions of the sensory axons.
|
TS28: main olfactory bulb external plexiform layer |
Strong |
Punctate |
2L
,
2D
,
2I
|
|
Specimen
3A,D:
embryonic day 14.5
(more )
Note: Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
Specimen
3A,D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: neural retina |
Present |
Regionally restricted |
3A
,
3D
|
Expression was high in retinal ganglion cells and their axons in the inner most layer of the retina.
|
TS22: optic nerve |
Present |
|
3A
,
3D
|
|
Specimen
3E,H:
embryonic day 17.5
(more )
Note: Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
Specimen
3E,H:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
|
Specimen
3I:
embryonic day 14.5
(more )
Specimen
3I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3J:
embryonic day 17.5
(more )
Specimen
3J:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3K:
postnatal day 0
(more )
Specimen
3K:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3L:
postnatal day 10
(more )
Specimen
3L:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cortical plate |
Present |
Regionally restricted |
4A
|
Expression was detected in the cortical plate of the cingulate cortex.
|
TS22: neocortex |
Present |
Regionally restricted |
4A
|
Expression was detected in the cortical plate of the cingulate cortex, but not in the underlying deeper layers of the cingulate cortex, including the intermediate zone and germinal layer.
|
TS22: fornix |
Present |
Regionally restricted |
4A
|
Expression was seen in axons projecting into the fornix.
|
Specimen
4B,E,H:
embryonic day 17.5
(more )
Note: Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
Specimen
4B,E,H:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebral cortex intermediate zone |
Strong |
Regionally restricted |
4B
|
Expression was strong in the intermediate zone underlying the cingulate cortex and more weakly expressed in the axons from the intermediate zone of the remaining neocortex.
|
TS25: corpus callosum |
Weak |
Regionally restricted |
4H
,
4E
,
4B
|
At the midline, the cingulate axons downregulate expression and diffusely course in the dorsal corpus callosum. Neocortical axons continue weak expression as crossing the midline in the ventral corpus callosum.
|
TS25: brain commissure |
Strong |
Regionally restricted |
4B
|
Robust expression was noted in the hippocampal commissure.
|
Specimen
4C,I,L:
postnatal day 0
(more )
Note: Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
Specimen
4C,I,L:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Eva1c; green - Gap43 (assay MGI:6147788).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebral cortex intermediate zone |
Strong |
Regionally restricted |
4C
|
Strong expression was detected in the intermediate zone of the cingulate cortex and neocortex.
|
TS27: cingulate cortex |
Strong |
Regionally restricted |
4C
|
Strong expression was detected in the intermediate zone of the cingulate cortex and neocortex.
|
TS27: neocortex |
Strong |
Regionally restricted |
4C
|
Strong expression was detected in the intermediate zone of the cingulate cortex and neocortex.
|
TS27: corpus callosum |
Strong |
Regionally restricted |
4L
,
4I
,
4C
|
Strong co-expression with Gap43-positive commissural axons through the dorsoventral depth of the corpus callosum.
|
TS27: hippocampal commissure |
Present |
|
4C
|
|
Specimen
4D:
postnatal day 10
(more )
Specimen
4D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5A:
embryonic day 14.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5B:
embryonic day 17.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5C:
postnatal day 0
(more )
Specimen
5C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5D:
postnatal day 10
(more )
Specimen
5D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|