Slc32a1tm2.1(flpo)Hze
Targeted Allele Detail
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| Symbol: |
Slc32a1tm2.1(flpo)Hze |
| Name: |
solute carrier family 32 (GABA vesicular transporter), member 1; targeted mutation 2.1, Hongkui Zeng |
| MGI ID: |
MGI:6150908 |
| Synonyms: |
Slc32a1-IRES2-FlpO-D, Slc32a1-IRES2-FlpO-Delta, Slc32a1-IRES2-FlpO-Deltaneo, VGAT-IRES2-FlpO-D |
| Gene: |
Slc32a1 Location: Chr2:158452687-158457668 bp, + strand Genetic Position: Chr2, 78.72 cM, cytoband H1-3
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| Alliance: |
Slc32a1tm2.1(flpo)Hze page
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Slc32a1tm2.1(flpo)Hze expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a partial Slc32a1 sequence spanning exon 1 through exon 2 up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a FlpO recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA::frt5::RNA splice acceptor::3'hygro-pA) and replace it with the recombined AttB/AttP site (AttL).
(J:260362)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Slc32a1
(mouse)
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| Original: |
J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22 |
| All: |
6 reference(s) |
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Analysis Tools
