Antibody:	anti-megalin
Detection system:	Secondary antibody coupled to Cy5

Structure	Level	Pattern	Image	Note
TS8: epiblast	Strong	Regionally restricted	1A	Expression is in the neural ectoderm extending from the future head region almost to the most caudal regions of the embryo.
TS8: ectoplacental cone	Present	Punctate	1A
TS8: extraembryonic visceral endoderm	Trace		1A

Structure	Level	Pattern	Image	Note
TS12: neural plate	Strong		1C
TS12: extraembryonic visceral endoderm	Present		1C
TS12: ectoplacental cone	Absent		1C
TS12: embryo endoderm	Absent		1C

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present		4B , 4A	Expression is at the apical surface.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present	Regionally restricted	4B	Expression is apical and basolateral.

Structure	Level	Pattern	Image	Note
TS12: blood island	Present	Regionally restricted	4C	Expression is in endothelial cells.
TS12: extraembryonic visceral endoderm	Present	Regionally restricted	4C inset , 4C	Expression is apical and basolateral.
TS12: blood	Absent		4C

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	5A	Expression is on the apical surface of the neural epithelium.
TS13: extraembryonic endoderm	Present	Patchy	5A
TS13: embryo endoderm	Absent		5A

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	5B	Expression is on the apical surface of the neural epithelium.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	5D	Expression is in cells that surround the ectoplacental cone.
TS13: ectoplacental cone	Trace		5D

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	5E	Expression is in clusters adjacent to the vascular sinuses (with little or no endothelial expression) and at the cell surface in cells that surround the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS15: extraembryonic endoderm	Strong		6A
TS15: ectoplacental cone	Present	Punctate	6A