Gene Expression Data
Assay Details
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Assay
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Reference:
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J:221192
Prasitsak T, et al., Foxc1 is required for early stage telencephalic vascular development. Dev Dyn. 2015 May;244(5):703-11
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6154609
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Gene symbol:
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Cspg4
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Gene name:
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chondroitin sulfate proteoglycan 4
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Antibody:
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anti-NG2 (AB5320)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1E:
embryonic day 11.0
(more  )
Note: Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
1E
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Expression in pericytes beginning to invade the ventral telencephalon along blood vessels, with some reaching the subventricular zone.
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Specimen
1F:
embryonic day 11.0; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
Specimen
1F:
(close )
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Genetic Background:
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involves: C57BL/6 * CHMU/Le
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Mutant Allele(s):
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Foxc1ch/Foxc1ch
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Age:
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embryonic day 11.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
1F
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Authors report mutant did not shown any delay in pericyte invasion or pericyte distribution.
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Specimen
1G:
embryonic day 11.5
(more )
Note: Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
1G
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Authors report more pericytes were observed in the subventricular zone at E11.0.
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Specimen
1H:
embryonic day 11.5; Foxc1ch/ Foxc1ch
(more )
Note: Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
Specimen
1H:
(close )
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Genetic Background:
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involves: C57BL/6 * CHMU/Le
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Mutant Allele(s):
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Foxc1ch/Foxc1ch
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - NG2 (Cspg4); red - CD31 (Pecam1 assay MGI:6154605); blue - Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
1H
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Authors report more pericytes were observed in the subventricular zone at E11.0.
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Analysis Tools