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Gad2tm1.1(flpo)Jnb
Targeted Allele Detail
Summary
Symbol: Gad2tm1.1(flpo)Jnb
Name: glutamic acid decarboxylase 2; targeted mutation 1.1, J Nicholas Betley
MGI ID: MGI:6156370
Synonyms: Gad2-IRES-FlpO
Gene: Gad2  Location: Chr2:22512262-22583889 bp, + strand  Genetic Position: Chr2, 15.15 cM, cytoband A-B
Alliance: Gad2tm1.1(flpo)Jnb page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:261454
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6J)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Gad2tm1.1(flpo)Jnb expression driven by 1 gene
 
Mutation details: The targeting vector containing a 10,389 bp genomic DNA fragment spanning exon 16 of mouse Gad2 and a cassette of IRES-FlpO-loxP2272-ACE-Cre POII NeoR-loxp2272 inserted between stop codon TAA and 3' UTR was introduced into ES cells. Positive ES clones were expanded for generation of chimeric mice which have gone through self excised NeoR cassette. (J:261454)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gad2 (mouse)
Summary of all recombinase alleles driven by Gad2.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gad2 Mutation:  60 strains or lines available
Notes
The targeting vector was electroporated into F1 hybrid of 129S6 x C57BL/6J ES cells derived by the Janelia Transgenic Facility (www.janelia.org).
References
Original:  J:261454 Alhadeff AL, et al., A Neural Circuit for the Suppression of Pain by a Competing Need State. Cell. 2018 Mar 22;173(1):140-152.e15
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
08/02/2024
MGI 6.24 		The Jackson Laboratory