Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:260419
Jiang X, et al., Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development. Gene Expr Patterns. 2018 Jan;27:93-98
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6192296
|
|
Gene symbol:
|
Mpdz
|
|
Gene name:
|
multiple PDZ domain crumbs cell polarity complex component
|
|
Antibody:
|
anti-MPDZ (42-2700)
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
Confocal images.
|
|
|
Results
|
Specimen
5 c8c:
embryonic day 2.5
(more  )
Note: Compacted 8-cell embryo.
Specimen
5 c8c:
(close  )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Compacted 8-cell embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
Regionally restricted |
5_c8c
,
5_c8c Merge
|
Expression is at tight junctions of outer blastomere contacts and at the apical membrane adjacent to tight junctions.
|
Specimen
5 Mo:
embryonic day 3.0
(more )
Note: Morula stage embryo.
Specimen
5 Mo:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Morula stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Regionally restricted |
5_Mo
,
5_Mo Merge
|
Expression is at tight junctions of outer blastomere contacts and at the apical membrane adjacent to tight junctions. Very weak expression is found at the basolateral membrane.
|
Specimen
5 Bl:
embryonic day 3.5
(more )
Note: Blastocyst stage embryo.
Specimen
5 Bl:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 3.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Blastocyst stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
Regionally restricted |
5_Bl Merge
,
5_Bl
|
Expression is at tight junctions of outer blastomere contacts and at the apical membrane adjacent to tight junctions. Very weak expression is found at the basolateral membrane.
|
Specimen
6D:
embryonic day 3.5
(more )
Note: Blastocyst stage embryo. Double labeled: green - Mpdz; red - Tjp1 (assay MGI:6192291).
Specimen
6D:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 3.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Blastocyst stage embryo. Double labeled: green - Mpdz; red - Tjp1 (assay MGI:6192291).
|
|
Analysis Tools