Fbxo2tm1.1(cre/ERT2)Hels
Targeted Allele Detail
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| Symbol: |
Fbxo2tm1.1(cre/ERT2)Hels |
| Name: |
F-box protein 2; targeted mutation 1.1, Stefan Heller |
| MGI ID: |
MGI:6194328 |
| Gene: |
Fbxo2 Location: Chr4:148245078-148250881 bp, + strand Genetic Position: Chr4, 78.68 cM
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| Alliance: |
Fbxo2tm1.1(cre/ERT2)Hels page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:266597
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Fbxo2tm1.1(cre/ERT2)Hels expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector was designed to insert a multicistronic reporter into the ATG start codon of the F-box protein 2 (Fbxo2) gene. Specifically, this vector includes (from 5' to 3') an H2B-Venus fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] with a C-terminal yellow fluorescent protein sequence), a hygromycin resistance gene flanked with viral P2A oligopeptides, a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain), a polyadenylation signal, and a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14tg2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Tg(EIIa-cre)C5379Lmgd mice on an FVB genetic background to remove the neo cassette.
(J:266597)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Fbxo2
(mouse)
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| Original: |
J:266597 Hartman BH, et al., Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Dev Biol. 2018 Nov 1;443(1):64-77 |
| All: |
1 reference(s) |
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Analysis Tools
