Gene Expression Data
Assay Details
Assay
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Reference:
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J:142873
Collins CA, et al., Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling. Dev Biol. 2008 Dec 1;324(1):55-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6196086
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Gene symbol:
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Fabp5
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Gene name:
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fatty acid binding protein 5, epidermal
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Antibody:
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FABP5 antibody
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Detection system:
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Alexa Fluor 488
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-Krt14 (MGI:6196068) may have been used in this assay.
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Results
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Specimen
2a:
embryonic day 13.5
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).v
Specimen
2a:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).v
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Structure |
Level |
Pattern |
Image |
Note |
TS21: back skin |
Absent |
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2a
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No expression in E13.5 skin.
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Specimen
2b:
embryonic day 15.5
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2b:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: epidermis |
Present |
Regionally restricted |
2b
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Expression in developing suprabasal layer of keratinocytes.
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Specimen
2c:
embryonic day 17.5
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2c:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: epidermis |
Present |
Regionally restricted |
2c
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Expression maintained in suprabasal epidermis. Expression largely absent from proliferating basal keratinocytes and upper-most differentiated layers of epidermis.
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Specimen
2d:
postnatal day 1
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2d:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: epidermis |
Present |
Regionally restricted |
2d
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Expression in suprabasal keratinocytes at all postnatal and adult stages examined. Expression in clusters of cells associated with tops of most mature primary follicles.
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Specimen
2e:
postnatal day 5
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2e:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epidermis suprabasal layer |
Present |
Regionally restricted |
2e
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Expression in suprabasal keratinocytes at all postnatal and adult stages examined.
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Specimen
2f:
embryonic day 17.5
(more )
Specimen
2f:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS25: vibrissa follicle |
Present |
Regionally restricted |
2f
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Expression in clusters of cells associated with whisker follicles.
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Specimen
2g:
postnatal day 5
(more )
Note: Double labeled: green - Fabp5; red - Mki67 (assay MGI:6196083).
Specimen
2g:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Mki67 (assay MGI:6196083).
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Specimen
2i:
postnatal day 1
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2i:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formalin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: hair follicle |
Present |
Regionally restricted |
2i
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Expression in clusters of cells associated with tops of most mature primary follicles, appearing to be germinal sebaceous glands.
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Specimen
2j:
postnatal day 1
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2j:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
postnatal day 1
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formalin
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Note:
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Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: tail |
Present |
Regionally restricted |
2j
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Expression in clusters of cells associated with tops of most mature primary follicles, appearing to be germinal sebaceous glands.
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Specimen
2k:
postnatal day 5
(more )
Note: Double labeled: green - Fabp5; red - Mki67 (assay MGI:6196083).
Specimen
2k:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Mki67 (assay MGI:6196083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: sebaceous gland |
Present |
Regionally restricted |
2k
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Authors state that expressing cells located in the sebaceous gland region were not proliferative.
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Specimen
2l:
postnatal day 42
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2l:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 42
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
|
Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epidermis suprabasal layer |
Present |
Regionally restricted |
2l
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Expression in suprabasal keratinocytes at all postnatal and adult stages examined. Expression in majority of differentiated cells.
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TS28: hair follicle |
Ambiguous |
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2l
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Weak or absent expression from adult telogen hair follicles.
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Specimen
2m:
postnatal day 42
(more )
Note: Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
Specimen
2m:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 42
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
|
Double labeled: green - Fabp5; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epidermis suprabasal layer |
Present |
Regionally restricted |
2m
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Prominent nuclear localization of expression in cells at interface between differentiating suprabasal layer and proliferating basal layer.
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Specimen
2n:
postnatal day 102
(more )
Note: Double labeled: green - Fabp5; red - Crabp1 (assay MGI:6196079).
Specimen
2n:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 102
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Crabp1 (assay MGI:6196079).
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Specimen
5a:
postnatal day 293
(more )
Note: Double labeled: green - Fabp5; red - Crabp1 (assay MGI:6196079).
Specimen
5a:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 293
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp5; red - Crabp1 (assay MGI:6196079).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: back skin |
Present |
Regionally restricted |
5a
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Authors report pattern of expression essentially similar to that seen in telogen skin of 42-day-old animals.
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Specimen
5e:
postnatal day 1
(more )
Specimen
5e:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
postnatal day 1
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formalin
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Structure |
Level |
Pattern |
Image |
Note |
TS27: tail |
Present |
Regionally restricted |
5e
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