Crhr1tm4(cre)Jde
Targeted Allele Detail
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| Symbol: |
Crhr1tm4(cre)Jde |
| Name: |
corticotropin releasing hormone receptor 1; targeted mutation 4, Jan Deussing |
| MGI ID: |
MGI:6201418 |
| Synonyms: |
Crhr1tZ-iCre |
| Gene: |
Crhr1 Location: Chr11:104023681-104066349 bp, + strand Genetic Position: Chr11, 67.77 cM
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| Alliance: |
Crhr1tm4(cre)Jde page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:264526
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| Parent Cell Line: |
TBV2 (ES Cell)
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| Strain of Origin: |
129S2/SvPas
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| Allele Type: |
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Targeted (Recombinase, Reporter) |
| Mutation: |
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Insertion
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Crhr1tm4(cre)Jde expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Crhr1 (MGI:88498) |
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Mutation details: Mice expressing Cre recombinase under the control of the Crhr1 promoter were generated by using a recombinase-mediated cassette exchange (RMCE) strategy. A construct with a docking site contained (5' to 3') an attP site, loxP site, FRT site, splice acceptor, T2A cleavage site, tau/lacZ/flag reporter cassette, FRT site, neo cassette, attP site, PGK promoter and FRT site, was inserted into intron 2. The attB site-flanked Crhr1-Cre recombinase expression unit encompassed, from 5' to 3', Crhr1 intron 2 to exon 3 fused to the Crhr1 cDNA covering exons 4-13, an ires-Cre cassette with a bGH-pA, and a frt site followed by a Pgk1 polyadenylation sequence and hygromycin resistance cassette, both in inverse orientation. phiC31 integrase-mediated cassette exchange resulted in insertion of the Crhr1-Cre expression unit into the right attP site. Resulting ES cells were used to generate chimeric mice, and mouse line. The tau-lacZ reporter and hygromycin selection cassette were removed by breeding to FLPeR mice.
(J:264526)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Crhr1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Crhr1 Mutation: |
28 strains or lines available
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| Original: |
J:264526 Dedic N, et al., Chronic CRH depletion from GABAergic, long-range projection neurons in the extended amygdala reduces dopamine release and increases anxiety. Nat Neurosci. 2018 Jun;21(6):803-807 |
| All: |
2 reference(s) |
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Analysis Tools
