Gene Expression Data
Assay Details
Assay
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Reference:
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J:198578
Chervenak AP, et al., Spatiotemporal expression of zic genes during vertebrate inner ear development. Dev Dyn. 2013 Jul;242(7):897-908
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6226134
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Gene symbol:
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Zic3
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Gene name:
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zinc finger protein of the cerebellum 3
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Probe:
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Zic3 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimens were postfixed in 4% paraformaldehyde, 0.2% glutaraldehyde.
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Results
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Specimen
6D:
embryonic day 9.5
(more )
Specimen
6D:
(close )
Genetic Background:
|
BALB/c
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6J:
embryonic day 10.5
(more )
Specimen
6J:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6P:
embryonic day 11.5
(more )
Specimen
6P:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
7D:
embryonic day 12.5
(more )
Specimen
7D:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: inner ear mesenchyme |
Present |
Regionally restricted |
7D
|
Expression is in cells surrounding the dorsal half of the developing inner ear and was strongest in cells adjacent to the dorsolateral region of the otic epithelium.
|
TS20: inner ear epithelium |
Absent |
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7D
|
|
Specimen
7D':
embryonic day 12.5
(more )
Specimen
7D':
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: inner ear mesenchyme |
Present |
Regionally restricted |
7D'
|
Expression is in cells surrounding the dorsal half of the developing inner ear and was strongest in cells adjacent to the dorsolateral region of the otic epithelium.
|
TS20: inner ear epithelium |
Absent |
|
7D'
|
|
Specimen
7J:
embryonic day 13.5
(more )
Specimen
7J:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: inner ear mesenchyme |
Present |
Regionally restricted |
7J
|
Expression is in cells surrounding the dorsal half of the developing inner ear and was strongest in cells adjacent to the dorsolateral region of the otic epithelium.
|
TS21: inner ear epithelium |
Absent |
|
7J
|
|
Specimen
7J':
embryonic day 13.5
(more )
Specimen
7J':
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: inner ear mesenchyme |
Present |
Regionally restricted |
7J'
|
Expression is in cells surrounding the dorsal half of the developing inner ear and was strongest in cells adjacent to the dorsolateral region of the otic epithelium.
|
TS21: inner ear epithelium |
Absent |
|
7J'
|
|
Specimen
not shown E12.5:
embryonic day 12.5
(more )
Specimen
not shown E12.5:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindbrain |
Present |
Regionally restricted |
|
Expression is dorsal.
|
Specimen
not shown E13.5:
embryonic day 13.5
(more )
Specimen
not shown E13.5:
(close )
Genetic Background:
|
BALB/c
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hindbrain |
Present |
Regionally restricted |
|
Expression is dorsal.
|
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