Tg(Tyr-Ctnnb1/EGFP)#Lru
Transgene Detail
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| Symbol: |
Tg(Tyr-Ctnnb1/EGFP)#Lru |
| Name: |
transgene insertion, Lionel Larue |
| MGI ID: |
MGI:6272337 |
| Synonyms: |
bcat*, bcatsta, Tyr::beta-cat*, Tyr::beta-cat-mut-nls-egfp |
| Transgene: |
Tg(Tyr-Ctnnb1/EGFP)#Lru Location: unknown
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| Alliance: |
Tg(Tyr-Ctnnb1/EGFP)#Lru page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Constitutively active, Inserted expressed sequence) |
| Mutations: |
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Insertion, Nucleotide substitutions
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Tg(Tyr-Ctnnb1/EGFP)#Lru expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Ctnnb1 (MGI:88276) |
mouse Tyr enh/promoter drives expression of a cDNA encoding a mutated form of beta-catenin tagged with EGFP and stabilized by four serine/threonine (S/T) to alanine (A) mutations in N-terminal region; a nuclear localization signal restricts protein to the nucleus |
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Mutation details: The mouse tyrosinase (Tyr) gene enhancer was fused to the Tyr promoter region to produce a 6.1-kb regulatory element driving the expression of a cDNA encoding a stabilized form of beta-catenin in which Ser33, Ser37, Ser45, and Thr41 were replaced by alanines (A33, A37, A41, A45). A nuclear localization signal (nls) and EGFP sequences were fused in frame to the 3' end of the mutated beta-catenin cDNA. An SV40 small T-antigen splice site and polyadenylation sequence were added to the 3' end of the construct to produce Tyr::beta-cat-mut-nls-egfp (bcatsta). This expression vector produces a constitutively activated nuclear form of the protein that is comparable in its activity to the mutated form of beta-catenin found in melanomas and other cancers. The transgene was confirmed to be specifically expressed in cells of the melanocyte lineage. Two independent lines (lines 1 and 2) were generated, both presenting a hypopigmented coat color phenotype. The level of transgene expression in melanocytes cultured from newborn skin was ~10% and 5% of that of the endogenous protein in lines 1 and 2, respectively. The lines are pooled, as denoted by the pound (#) sign.
(J:127315)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:127315 Delmas V, et al., Beta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development. Genes Dev. 2007 Nov 15;21(22):2923-35 |
| All: |
5 reference(s) |
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Analysis Tools
