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Tg(Gfap-flpo)62Thara
Transgene Detail
Summary
Symbol: Tg(Gfap-flpo)62Thara
Name: transgene insertion 62, Toshiro Hara
MGI ID: MGI:6294098
Synonyms: GFAP-FLPo
Transgene: Tg(Gfap-flpo)62Thara  Location: unknown  
Alliance: Tg(Gfap-flpo)62Thara page
Transgene
origin
Strain of Origin:  B6D2F1
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Tg(Gfap-flpo)62Thara expression driven by 1 gene
 
Mutation details: A transgene consist of an 15 kbp mouse glial fibrillary acidic protein (Gfap) sequence including its promoter region, regulatory elements, exons, introns and 3' UTR, as well as having a point mutation (ATG to TTG) in the Gfap start codon site to prevent GFAP expression from the transgene with an optimized FLP recombinase (flpo) followed by BGH polyA integrated into the Gfap first exon was used. The final transgene was used for pronuclear injection into B6D2F1/J x B6D2F1/J embryos. Founder animals were bred to C57BL/6J inbred mice for germline transmission. Founder line #62 was identified to have flpo expression predominantly in brain, and had the least amount of non-brain expression. (J:278165)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gfap (mouse)
Summary of all recombinase alleles driven by Gfap.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:278165 Hara T, et al., Modeling Gliomas Using Two Recombinases. Cancer Res. 2019 Aug 1;79(15):3983-3991
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory