Dbh<tm3.2(cre)Pjen> Targeted Allele Detail MGI Mouse (MGI:6305870)

Dbh^{tm3.2(cre)Pjen}
Targeted Allele Detail

Summary

Symbol:	Dbh^{tm3.2(cre)Pjen}
Name:	dopamine beta hydroxylase; targeted mutation 3.2, Patricia Jensen
MGI ID:	MGI:6305870
Synonyms:	Dbh^cre
Gene:	Dbh Location: Chr2:27055519-27073216 bp, + strand Genetic Position: Chr2, 19.29 cM
Alliance:	Dbh^{tm3.2(cre)Pjen} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:285301
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Mutation
description

Allele Type:

Targeted (Recombinase)

Mutation:

Insertion

Dbh^{tm3.2(cre)Pjen} expression driven by 1 gene

Mutation details: A rox-flanked transcriptional stop cassette, cre cDNA, rabbit beta-globin polyadenylation cassette, and attB/attP-flanked neomycin resistance cassette were inserted into the Dbh locus, immediately following the start codon by homologous recombination. A recombinant clone was transiently transfected with pPGKPhiC31obpa to excise the neomycin resistance cassette before cells were injected into blastocysts to produce chimeric mice. Following germline transmission, heterozygotes were crossed with Dre expressing mice to remove the rox-flanked stop cassette. (J:285301)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dbh (mouse) Summary of all recombinase alleles driven by Dbh. Comparative matrix view of recombinase activities Alliance mouse Dbh gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Dbh Mutation:	17 strains or lines available

References

Original:	J:285301 Tillage RP, et al., Elimination of galanin synthesis in noradrenergic neurons reduces galanin in select brain areas and promotes active coping behaviors. Brain Struct Funct. 2020;225(2):785-803
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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