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Gene Expression Data
Assay Details
Assay
Reference: J:275336 Sulistomo HW, et al., Formin homology 2 domain-containing 3 (Fhod3) controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction. J Biol Chem. 2019 Feb 22;294(8):2924-2934
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6314401
Gene symbol: Fhod3
Gene name: formin homology 2 domain containing 3
Antibody: Anti-Fhod3 (650-802)
Detection system: Alexa Fluor 488
Assay notes: Fixation was with 3.7% paraformaldehyde.
Results
Specimen 5C +/+ Fhod3 upper: embryonic day 9.5 (more )
Note: Double labeled: green - Fhod3; red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 5C +/+ upper Fhod3 , 5C +/+ upper merge Authors state apical acumulation of endogenous Fhod was detected specifically in wild type embryos.
Specimen 5C +/+ Fhod3 lower: embryonic day 9.5 (more )
Note: Double labeled: green - Fhod3; red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present Regionally restricted 5C +/+ lower merge , 5C +/+ lower Fhod3 Authors state apical acumulation of endogenous Fhod was detected specifically in wild type embryos.
Specimen 5C -/- Fhod3 upper: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - Fhod3; red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Ambiguous 5C -/- upper merge , 5C -/- upper Fhod3
Specimen 5C -/- Fhod3 lower: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Note: Double labeled: green - Fhod3; red - Phalloidin.
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Ambiguous 5C -/- lower merge , 5C -/- lower Fhod3
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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