Gene Expression Data
Assay Details
Assay
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Reference:
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J:45249
Volpe MV, et al., Hoxb-5 expression in the developing mouse lung suggests a role in branching morphogenesis and epithelial cell fate. Histochem Cell Biol. 1997 Dec;108(6):495-504
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6316622
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Gene symbol:
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Hoxb5
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Gene name:
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homeobox B5
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Antibody:
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Anti-Hoxb5
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to alkaline phosphatase
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Results
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Specimen
1B:
embryonic day 13.5
(more )
Specimen
1B:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1D:
embryonic day 15.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 15.5
|
Sex:
|
Male
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung |
Present |
Regionally restricted |
1D
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Specific staining was present in nuclei of subepithelial fibroblasts and adjacent columnar epithelial cells of branching airways. Mesenchymal cells distal from developing airways were devoid of specific staining.
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TS23: lung epithelium |
Present |
|
1D
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TS23: bronchiole |
Present |
Regionally restricted |
1D
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Staining was particularly prominent at airway branch points of terminal airways with columnar epithelia.
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TS23: trachea epithelium |
Absent |
|
1D
|
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Specimen
2B:
embryonic day 16.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: terminal bronchiole |
Present |
|
2B
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Specific staining was seen in nuclei of columnar and cuboidal epithelial cells of terminal conducting airways and in adjacent subepithelial fibroblasts.
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TS24: terminal bronchiole epithelium |
Present |
|
2B
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TS24: bronchiole |
Present |
Regionally restricted |
2B
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Positive staining was detected in nuclei of low cuboidal epithelia at branch points just before the entrance to developing alveolar ducts.
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TS24: alveolar system |
Absent |
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2B
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Staining was absent from flattened epithelial cells of alveolar ducts and early alveolar sacs.
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TS24: trachea epithelium |
Absent |
|
2B
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Specimen
2C:
embryonic day 17.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 17.5
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Sex:
|
Male
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2D:
postnatal day 1
(more )
Specimen
2D:
(close )
Genetic Background:
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Swiss Webster
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Age:
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postnatal day 1
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Sex:
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Male
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: terminal bronchiole epithelium |
Present |
Non-Uniform |
2D
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Staining was seen in some but not all epithelial cells of terminal conducting airways.
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TS27: lobar bronchus |
Present |
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2D
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Specific staining was present in some subepithelial fibroblasts and isolated epithelial cells of large conducting airways and terminal bronchioles.
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TS27: terminal bronchiole |
Present |
|
2D
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Specific staining was present in some subepithelial fibroblasts and isolated epithelial cells of large conducting airways and terminal bronchioles.
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TS27: alveolus epithelium |
Absent |
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2D
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Epithelial cells of early alveolar sacs continued to be devoid of specific staining.
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Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: lobar bronchus epithelium |
Present |
Single cells |
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Staining was seen rarely in isolated conducting airway epithelial cells.
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