Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:266902
Rafipay A, et al., Expression analysis of limb element markers during mouse embryonic development. Dev Dyn. 2018 Nov;247(11):1217-1226
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6342222
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Gene symbol:
|
Myod1
|
Gene name:
|
myogenic differentiation 1
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Probe:
|
Myod1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
3A:
embryonic day 13.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb muscle |
Strong |
Regionally restricted |
3A
|
Strong staining was detected in stylopod and zeugopod developing muscle blocks.
|
TS21: hand |
Weak |
|
3A
|
|
Specimen
3A':
embryonic day 13.5
(more )
Specimen
3A':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb muscle |
Strong |
Regionally restricted |
3A'
|
Strong staining was detected in stylopod and zeugopod developing muscle blocks.
|
TS21: hand |
Weak |
|
3A'
|
|
Specimen
3B:
embryonic day 13.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hindlimb muscle |
Strong |
Regionally restricted |
3B
|
Strong staining was detected in stylopod and zeugopod developing muscle blocks.
|
TS21: foot |
Weak |
|
3B
|
|
Specimen
3B':
embryonic day 13.5
(more )
Specimen
3B':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: hindlimb muscle |
Strong |
Regionally restricted |
3B'
|
Strong staining was detected in stylopod and zeugopod developing muscle blocks.
|
TS21: foot |
Weak |
|
3B'
|
|
Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: zeugopod |
Strong |
Regionally restricted |
3C
|
Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
|
TS22: forelimb muscle |
Strong |
Regionally restricted |
3C
|
Strong expression was detected in bands of developing forearm muscle.
|
TS22: hand |
Present |
Regionally restricted |
3C
|
The handplate showed expression in muscle bands from the center radiating to digit bases.
|
TS22: wrist |
Present |
Regionally restricted |
3C
|
A thin band of expression through the middle of the wrist connected expression in the zeugopod to the autopod in the forelimb.
|
Specimen
3C':
embryonic day 14.5
(more )
Specimen
3C':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: zeugopod |
Strong |
Regionally restricted |
3C'
|
Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
|
TS22: forelimb muscle |
Strong |
Regionally restricted |
3C'
|
Strong expression was detected in bands of developing forearm muscle.
|
TS22: hand |
Present |
Regionally restricted |
3C'
|
The handplate showed expression in muscle bands from the center radiating to digit bases.
|
TS22: wrist |
Present |
Regionally restricted |
3C'
|
A thin band of expression through the middle of the wrist connected expression in the zeugopod to the autopod in the forelimb.
|
Specimen
3D:
embryonic day 14.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: zeugopod |
Strong |
Regionally restricted |
3D
|
Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
|
TS22: hindlimb muscle |
Strong |
Regionally restricted |
3D
|
Strong expression was detected in bands of developing leg muscle.
|
TS22: foot |
Present |
Regionally restricted |
3D
|
The footplate showed expression in muscle bands from the center radiating to digit bases.
|
Specimen
3D':
embryonic day 14.5
(more )
Specimen
3D':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: zeugopod |
Strong |
Regionally restricted |
3D'
|
Strong expression was detected in the proximal zeugopod. Expression in the forelimb zeugopod was stronger than in the hindlimb zeugopod.
|
TS22: hindlimb muscle |
Strong |
Regionally restricted |
3D'
|
Strong expression was detected in bands of developing leg muscle.
|
TS22: foot |
Present |
Regionally restricted |
3D'
|
The footplate showed expression in muscle bands from the center radiating to digit bases.
|
Specimen
3E:
embryonic day 15.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: autopod |
Strong |
Regionally restricted |
3E
,
3G
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: zeugopod |
Strong |
Regionally restricted |
3E
,
3G
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: wrist |
Strong |
Regionally restricted |
3G
,
3E
|
The band of expression connecting the zeugopod and autopod was stronger in the forelimb of E15.5 compared to E14.5.
|
TS23: forelimb muscle |
Strong |
Regionally restricted |
3G
,
3E
|
Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.
|
Specimen
3E':
embryonic day 15.5
(more )
Specimen
3E':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: autopod |
Strong |
Regionally restricted |
3E'
,
3G'
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: zeugopod |
Strong |
Regionally restricted |
3E'
,
3G'
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: wrist |
Strong |
Regionally restricted |
3E'
,
3G'
|
The band of expression connecting the zeugopod and autopod was stronger in the forelimb of E15.5 compared to E14.5.
|
TS23: forelimb muscle |
Strong |
Regionally restricted |
3E'
,
3G'
|
Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.
|
Specimen
3F:
embryonic day 15.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: autopod |
Strong |
Regionally restricted |
3H
,
3F
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: zeugopod |
Strong |
Regionally restricted |
3H
,
3F
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: hindlimb muscle |
Strong |
Regionally restricted |
3H
,
3F
|
Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.
|
Specimen
3F':
embryonic day 15.5
(more )
Specimen
3F':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: autopod |
Strong |
Regionally restricted |
3F'
,
3H'
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: zeugopod |
Strong |
Regionally restricted |
3F'
,
3H'
|
Expression at E15.5 was similar to that at E14.5.
|
TS23: hindlimb muscle |
Strong |
Regionally restricted |
3H'
,
3F'
|
Expression of Myod1 demonstrated the definition and organization of fibers into defined muscle sets by E15.5.
|
|